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DOI	10.1073/pnas.1703834114
	Virus found in a boreal lake links ssDNA and dsDNA viruses
	Laanto E.; Mäntynen S.; De Colibus L.; Marjakangas J.; Gillum A.; Stuart D.I.; Ravantti J.J.; Huiskonen J.T.; Sundberg L.-R.
发表日期	2017
ISSN	0027-8424
起始页码	8378
结束页码	8383
卷号	114 期号:31
英文摘要	Viruses have impacted the biosphere in numerous ways since the dawn of life. However, the evolution, genetic, structural, and taxonomic diversity of viruses remain poorly understood, in part because sparse sampling of the virosphere has concentrated mostly on exploring the abundance and diversity of dsDNA viruses. Furthermore, viral genomes are highly diverse, and using only the current sequence-based methods for classifying viruses and studying their phylogeny is complicated. Here we describe a virus, FLiP (Flavobacterium-infecting, lipid-containing phage), with a circular ssDNA genome and an internal lipid membrane enclosed in the icosahedral capsid. The 9,174-nt-long genome showed limited sequence similarity to other known viruses. The genetic data imply that this virus might use replication mechanisms similar to those found in other ssDNA replicons. However, the structure of the viral major capsid protein, elucidated at near-atomic resolution using cryo-electron microscopy, is strikingly similar to that observed in dsDNA viruses of the PRD1–adenovirus lineage, characterized by a major capsid protein bearing two β-barrels. The strong similarity between FLiP and another member of the structural lineage, bacteriophage PM2, extends to the capsid organization (pseudo T = 21 dextro) despite the difference in the genetic material packaged and the lack of significant sequence similarity. © 2017, National Academy of Sciences. All rights reserved.
英文关键词	Cryo-electron microscopy; Flavobacterium; Genome; Lipids; Structure
语种	英语
scopus关键词	capsid protein; double stranded DNA; fresh water; RNA 16S; single stranded DNA; sucrose; virus DNA; capsid protein; single stranded DNA; Article; controlled study; double-stranded DNA virus; electron microscopy; lake; microbial community; nonhuman; priority journal; replicon; RNA sequence; single-stranded DNA virus; sucrose gradient; tandem mass spectrometry; virion; virus genome; virus isolation; virus purification; classification; DNA virus; Enterobacteria phage PRD1; Flavobacterium; genetics; isolation and purification; lake; metabolism; protein conformation; virology; virus capsid; Bacteriophage PRD1; Capsid; Capsid Proteins; DNA Viruses; DNA, Single-Stranded; Flavobacterium; Genome, Viral; Lakes; Protein Conformation
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160606
作者单位	Laanto, E., Centre of Excellence in Biological Interactions, Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, 40014, Finland, Nanoscience Center, University of Jyväskylä, Jyväskylä, 40014, Finland; Mäntynen, S., Centre of Excellence in Biological Interactions, Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, 40014, Finland, Nanoscience Center, University of Jyväskylä, Jyväskylä, 40014, Finland; De Colibus, L., Division of Structural Biology, Welcome Trust Centre for Human Genetics, University of Oxford, Oxford, OX3 7BN, United Kingdom; Marjakangas, J., Centre of Excellence in Biological Interactions, Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, 40014, Finland, Nanoscience Center, University of Jyväskylä, Jyväskylä, 40014, Finland; Gillum, A., Division of Structural Biology, Welcome Trust Centre for Human Genetics, University of Oxford, Oxford, OX3 7BN, United Kingdom; Stuart, D.I., Divi...
推荐引用方式 GB/T 7714	Laanto E.,Mäntynen S.,De Colibus L.,et al. Virus found in a boreal lake links ssDNA and dsDNA viruses[J],2017,114(31).
APA	Laanto E..,Mäntynen S..,De Colibus L..,Marjakangas J..,Gillum A..,...&Sundberg L.-R..(2017).Virus found in a boreal lake links ssDNA and dsDNA viruses.Proceedings of the National Academy of Sciences of the United States of America,114(31).
MLA	Laanto E.,et al."Virus found in a boreal lake links ssDNA and dsDNA viruses".Proceedings of the National Academy of Sciences of the United States of America 114.31(2017).