气候变化领域集成服务门户(CCPortal): Cytoplasmic MTOCs control spindle orientation for asymmetric cell division in plants

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DOI	10.1073/pnas.1713925114
	Cytoplasmic MTOCs control spindle orientation for asymmetric cell division in plants
	Kosetsu K.; Murata T.; Yamada M.; Nishina M.; Boruc J.; Hasebe M.; Van Damme D.; Goshima G.
发表日期	2017
ISSN	0027-8424
起始页码	E8847
结束页码	E8854
卷号	114 期号:42
英文摘要	Proper orientation of the cell division axis is critical for asymmetric cell divisions that underpin cell differentiation. In animals, centrosomes are the dominant microtubule organizing centers (MTOC) and play a pivotal role in axis determination by orienting the mitotic spindle. In land plants that lack centrosomes, a critical role of a microtubular ring structure, the preprophase band (PPB), has been observed in this process; the PPB is required for orienting (before prophase) and guiding (in telophase) the mitotic apparatus. However, plants must possess additional mechanisms to control the division axis, as certain cell types or mutants do not form PPBs. Here, using live imaging of the gametophore of the moss Physcomitrella patens, we identified acentrosomal MTOCs, which we termed “gametosomes,” appearing de novo and transiently in the prophase cytoplasm independent of PPB formation. We show that gametosomes are dispensable for spindle formation but required for metaphase spindle orientation. In some cells, gametosomes appeared reminiscent of the bipolar MT “polar cap” structure that forms transiently around the prophase nucleus in angiosperms. Specific disruption of the polar caps in tobacco cells misoriented the metaphase spindles and frequently altered the final division plane, indicating that they are functionally analogous to the gametosomes. These results suggest a broad use of transient MTOC structures as the spindle orientation machinery in plants, compensating for the evolutionary loss of centrosomes, to secure the initial orientation of the spindle in a spatial window that allows subsequent fine-tuning of the division plane axis by the guidance machinery. © 2017, National Academy of Sciences. All rights reserved.
英文关键词	Asymmetric cell division; MTOC; Physcomitrella patens; Spindle orientation; γ-tubulin
语种	英语
scopus关键词	F actin; gamma tubulin; actin; microtubule associated protein; plant protein; tubulin; anaphase; angiosperm; Article; asymmetric cell division; centrosome; controlled study; cytoplasm; depolymerization; metaphase; microtubule organizing center; mitosis spindle; nonhuman; phragmoplast; Physcomitrella patens; plant cell; plant structures; priority journal; prophase; asymmetric cell division; Bryopsida; cytology; cytoplasm; genetics; metabolism; microtubule; procedures; spindle apparatus; time lapse imaging; tobacco; transgenic plant; ultrastructure; Actins; Asymmetric Cell Division; Bryopsida; Cytoplasm; Microtubule-Associated Proteins; Microtubules; Plant Cells; Plant Proteins; Plants, Genetically Modified; Prophase; Spindle Apparatus; Time-Lapse Imaging; Tobacco; Tubulin
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160585
作者单位	Kosetsu, K., Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya, 464-8602, Japan, Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, 9052, Belgium, Center for Plant Systems Biology, VIB, Ghent, 9052, Belgium; Murata, T., Division of Evolutionary Biology, National Institute for Basic Biology, Myodaiji-cho, Okazaki, 444-8585, Japan, Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies (SOKENDAI), Myodaiji-cho, Okazaki, 444-8585, Japan; Yamada, M., Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya, 464-8602, Japan; Nishina, M., Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya, 464-8602, Japan; Boruc, J., Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, 9052, Belgium, Center for Plant Systems Biology, VIB, Ghent, 9052, Belgium; Hasebe, M., Division of Evolutionary Biolog...
推荐引用方式 GB/T 7714	Kosetsu K.,Murata T.,Yamada M.,et al. Cytoplasmic MTOCs control spindle orientation for asymmetric cell division in plants[J],2017,114(42).
APA	Kosetsu K..,Murata T..,Yamada M..,Nishina M..,Boruc J..,...&Goshima G..(2017).Cytoplasmic MTOCs control spindle orientation for asymmetric cell division in plants.Proceedings of the National Academy of Sciences of the United States of America,114(42).
MLA	Kosetsu K.,et al."Cytoplasmic MTOCs control spindle orientation for asymmetric cell division in plants".Proceedings of the National Academy of Sciences of the United States of America 114.42(2017).