气候变化领域集成服务门户(CCPortal): Nonequilibrium fluctuations of lipid membranes by the rotating motor protein F1F0-ATP synthase

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DOI	10.1073/pnas.1701207114
	Nonequilibrium fluctuations of lipid membranes by the rotating motor protein F1F0-ATP synthase
	Almendro-Vedia V.G.; Natale P.; Mell M.; Bonneau S.; Monroy F.; Joubert F.; López-Montero I.
发表日期	2017
ISSN	0027-8424
起始页码	11291
结束页码	11296
卷号	114 期号:43
英文摘要	ATP synthase is a rotating membrane protein that synthesizes ATP through proton-pumping activity across the membrane. To unveil the mechanical impact of this molecular active pump on the bending properties of its lipid environment, we have functionally reconstituted the ATP synthase in giant unilamellar vesicles and tracked the membrane fluctuations by means of flickering spectroscopy. We find that ATP synthase rotates at a frequency of about 20 Hz, promoting large nonequilibrium deformations at discrete hot spots in lipid vesicles and thus inducing an overall membrane softening. The enhanced nonequilibrium fluctuations are compatible with an accumulation of active proteins at highly curved membrane sites through a curvature-protein coupling mechanism that supports the emergence of collective effects of rotating ATP synthases in lipid membranes.
英文关键词	Active membranes; Biological nanorotors; Flickering spectroscopy; Giant vesicles; Mechanical properties
语种	英语
scopus关键词	proton transporting adenosine triphosphate synthase; adenosine triphosphate; Escherichia coli protein; Escherichia coli Proton-Translocating ATPase; fluorescent dye; liposome; proton transporting adenosine triphosphatase; recombinant protein; rhodamine 123; valinomycin; Article; Escherichia coli; giant unilamellar vesicle; lipid membrane; lipid vesicle; membrane model; priority journal; spectroscopy; surface tension; biosynthesis; cell membrane; chemistry; drug effect; genetics; lipid bilayer; metabolism; pH; video microscopy; Adenosine Triphosphate; Bacterial Proton-Translocating ATPases; Cell Membrane; Escherichia coli Proteins; Fluorescent Dyes; Hydrogen-Ion Concentration; Lipid Bilayers; Microscopy, Video; Recombinant Proteins; Rhodamine 123; Unilamellar Liposomes; Valinomycin
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160584
作者单位	Almendro-Vedia, V.G., Departamento Química Física I, Universidad Complutense de Madrid, Madrid, 28040, Spain, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), Madrid, 28041, Spain; Natale, P., Departamento Química Física I, Universidad Complutense de Madrid, Madrid, 28040, Spain, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), Madrid, 28041, Spain; Mell, M., Departamento Química Física I, Universidad Complutense de Madrid, Madrid, 28040, Spain; Bonneau, S., Laboratoire Jean Perrin, CNRS, Université Pierre et Marie Curie, Paris, 75005, France; Monroy, F., Departamento Química Física I, Universidad Complutense de Madrid, Madrid, 28040, Spain, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), Madrid, 28041, Spain; Joubert, F., Laboratoire Jean Perrin, CNRS, Université Pierre et Marie Curie, Paris, 75005, France; López-Montero, I., Departamento Química Física I, Universidad Complutense de Madrid, Madrid, 28040, Spain, Instituto de Investigación Sa...
推荐引用方式 GB/T 7714	Almendro-Vedia V.G.,Natale P.,Mell M.,et al. Nonequilibrium fluctuations of lipid membranes by the rotating motor protein F1F0-ATP synthase[J],2017,114(43).
APA	Almendro-Vedia V.G..,Natale P..,Mell M..,Bonneau S..,Monroy F..,...&López-Montero I..(2017).Nonequilibrium fluctuations of lipid membranes by the rotating motor protein F1F0-ATP synthase.Proceedings of the National Academy of Sciences of the United States of America,114(43).
MLA	Almendro-Vedia V.G.,et al."Nonequilibrium fluctuations of lipid membranes by the rotating motor protein F1F0-ATP synthase".Proceedings of the National Academy of Sciences of the United States of America 114.43(2017).