气候变化领域集成服务门户(CCPortal): Methyl-compound use and slow growth characterize microbial life in 2-km-deep subseafloor coal and shale beds

CCPortal

DOI	10.1073/pnas.1707525114
	Methyl-compound use and slow growth characterize microbial life in 2-km-deep subseafloor coal and shale beds
	Trembath-Reichert E.; Morono Y.; Ijiri A.; Hoshino T.; Dawson K.S.; Inagaki F.; Orphan V.J.
发表日期	2017
ISSN	0027-8424
起始页码	E9206
结束页码	E9215
卷号	114 期号:44
英文摘要	The past decade of scientific ocean drilling has revealed seemingly ubiquitous, slow-growing microbial life within a range of deep biosphere habitats. Integrated Ocean Drilling Program Expedition 337 expanded these studies by successfully coring Miocene-aged coal beds 2 km below the seafloor hypothesized to be “hot spots” for microbial life. To characterize the activity of coal-associated microorganisms from this site, a series of stable isotope probing (SIP) experiments were conducted using intact pieces of coal and overlying shale incubated at in situ temperatures (45 °C). The 30-month SIP incubations were amended with deuterated water as a passive tracer for growth and different combinations of 13C- or 15N-labeled methanol, methylamine, and ammonium added at low (micromolar) concentrations to investigate methylotrophy in the deep subseafloor biosphere. Although the cell densities were low (50–2,000 cells per cubic centimeter), bulk geochemical measurements and single-cell–targeted nanometer-scale secondary ion mass spectrometry demonstrated active metabolism of methylated substrates by the thermally adapted microbial assemblage, with differing substrate utilization profiles between coal and shale incubations. The conversion of labeled methylamine and methanol was predominantly through heterotrophic processes, with only minor stimulation of methanogenesis. These findings were consistent with in situ and incubation 16S rRNA gene surveys. Microbial growth estimates in the incubations ranged from several months to over 100 y, representing some of the slowest direct measurements of environmental microbial biosynthesis rates. Collectively, these data highlight a small, but viable, deep coal bed biosphere characterized by extremely slow-growing heterotrophs that can utilize a diverse range of carbon and nitrogen substrates. © 2017, National Academy of Sciences. All rights reserved.
英文关键词	Coal bed biosphere; Microbial generation time; NanoSIMS; Stable isotope probing; Subseafloor life
语种	英语
scopus关键词	ammonia; carbon; coal; methanol; methyl group; methylamine; nitrogen; stable isotope; coal; isotope; sea water; Article; biosphere; biosynthesis; cell density; geochemical analysis; habitat; heterotroph; life; metabolism; methanogenesis; methylation; microbial growth; Miocene; priority journal; sea; secondary ion mass spectrometry; temperature; aquatic species; biomass; ecosystem; growth, development and aging; microbiology; procedures; sediment; Aquatic Organisms; Biomass; Coal; Ecosystem; Geologic Sediments; Isotopes; Methanol; Methylamines; Seawater; Spectrometry, Mass, Secondary Ion
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160582
作者单位	Trembath-Reichert, E., Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125, United States; Morono, Y., Geomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Monobe B200, Nankoku, Kochi 783-8502, Japan, Geobiotechnology Group, Research and Development Center for Submarine Resources, JAMSTEC, Monobe B200, Nankoku, Kochi 783-8502, Japan; Ijiri, A., Geomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Monobe B200, Nankoku, Kochi 783-8502, Japan, Geobiotechnology Group, Research and Development Center for Submarine Resources, JAMSTEC, Monobe B200, Nankoku, Kochi 783-8502, Japan; Hoshino, T., Geomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Monobe B200, Nankoku, Kochi 783-8502, Japan, Geobiotechnology Group, Research and Developm...
推荐引用方式 GB/T 7714	Trembath-Reichert E.,Morono Y.,Ijiri A.,et al. Methyl-compound use and slow growth characterize microbial life in 2-km-deep subseafloor coal and shale beds[J],2017,114(44).
APA	Trembath-Reichert E..,Morono Y..,Ijiri A..,Hoshino T..,Dawson K.S..,...&Orphan V.J..(2017).Methyl-compound use and slow growth characterize microbial life in 2-km-deep subseafloor coal and shale beds.Proceedings of the National Academy of Sciences of the United States of America,114(44).
MLA	Trembath-Reichert E.,et al."Methyl-compound use and slow growth characterize microbial life in 2-km-deep subseafloor coal and shale beds".Proceedings of the National Academy of Sciences of the United States of America 114.44(2017).