气候变化领域集成服务门户(CCPortal): Bacteriocyte cell death in the pea aphid/Buchnera symbiotic system

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DOI	10.1073/pnas.1720237115
	Bacteriocyte cell death in the pea aphid/Buchnera symbiotic system
	Simonet P.; Gaget K.; Balmand S.; Lopes M.R.; Parisot N.; Buhler K.; Duport G.; Vulsteke V.; Febvay G.; Heddi A.; Charles H.; Callaerts P.; Calevro F.
发表日期	2018
ISSN	0027-8424
起始页码	E1819
结束页码	E1827
卷号	115 期号:8
英文摘要	Symbiotic associations play a pivotal role in multicellular life by facilitating acquisition of new traits and expanding the ecological capabilities of organisms. In insects that are obligatorily dependent on intracellular bacterial symbionts, novel host cells (bacteriocytes) or organs (bacteriomes) have evolved for harboring beneficial microbial partners. The processes regulating the cellular life cycle of these endosymbiont-bearing cells, such as the cell-death mechanisms controlling their fate and elimination in response to host physiology, are fundamental questions in the biology of symbiosis. Here we report the discovery of a cell-death process involved in the degeneration of bacteriocytes in the hemipteran insect Acyrthosiphon pisum. This process is activated progressively throughout aphid adulthood and exhibits morphological features distinct from known cell-death pathways. By combining electron microscopy, immunohistochemistry, and molecular analyses, we demonstrated that the initial event of bacteriocyte cell death is the cytoplasmic accumulation of nonautophagic vacuoles, followed by a sequence of cellular stress responses including the formation of autophagosomes in intervacuolar spaces, activation of reactive oxygen species, and Buchnera endosymbiont degradation by the lysosomal system. We showed that this multi-step cell-death process originates from the endoplasmic reticulum, an organelle exhibiting a unique reticular network organization spread throughout the entire cytoplasm and surrounding Buchnera aphidicola endosymbionts. Our findings provide insights into the cellular and molecular processes that coordinate eukaryotic host and endosymbiont homeostasis and death in a symbiotic system and shed light on previously unknown aspects of bacteriocyte biological functioning. © 2018 National Academy of Sciences. All Rights Reserved.
英文关键词	Acyrthosiphon pisum; Bacteriocytes; Buchnera aphidicola; Cell death; Symbiosis
语种	英语
scopus关键词	reactive oxygen metabolite; adult; animal cell; animal tissue; Article; autophagosome; bacteriocyte; Buchnera aphidicola; cell death; cell structure; cell vacuole; cellular stress response; controlled study; cytoplasm; electron microscopy; endoplasmic reticulum; endosymbiont; genetic procedures; giant cell; immunohistochemistry; lysosome; nonhuman; pea aphid; priority journal; symbiosis; animal; aphid; Buchnera; cell death; microbiology; physiology; symbiosis; Animals; Aphids; Buchnera; Cell Death; Lysosomes; Symbiosis
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160542
作者单位	Simonet, P., UMR0203, Biologie Fonctionnelle, Insectes et Interactions BF2I, Institut National des Sciences Appliquées (INSA-Lyon), Institut National de la Recherche Agronomique (INRA), University of Lyon, Villeurbanne, F-69621, France; Gaget, K., UMR0203, Biologie Fonctionnelle, Insectes et Interactions BF2I, Institut National des Sciences Appliquées (INSA-Lyon), Institut National de la Recherche Agronomique (INRA), University of Lyon, Villeurbanne, F-69621, France; Balmand, S., UMR0203, Biologie Fonctionnelle, Insectes et Interactions BF2I, Institut National des Sciences Appliquées (INSA-Lyon), Institut National de la Recherche Agronomique (INRA), University of Lyon, Villeurbanne, F-69621, France; Lopes, M.R., UMR0203, Biologie Fonctionnelle, Insectes et Interactions BF2I, Institut National des Sciences Appliquées (INSA-Lyon), Institut National de la Recherche Agronomique (INRA), University of Lyon, Villeurbanne, F-69621, France; Parisot, N., UMR0203, Biologie Fonctionnelle, Insectes et Interactions BF2...
推荐引用方式 GB/T 7714	Simonet P.,Gaget K.,Balmand S.,et al. Bacteriocyte cell death in the pea aphid/Buchnera symbiotic system[J],2018,115(8).
APA	Simonet P..,Gaget K..,Balmand S..,Lopes M.R..,Parisot N..,...&Calevro F..(2018).Bacteriocyte cell death in the pea aphid/Buchnera symbiotic system.Proceedings of the National Academy of Sciences of the United States of America,115(8).
MLA	Simonet P.,et al."Bacteriocyte cell death in the pea aphid/Buchnera symbiotic system".Proceedings of the National Academy of Sciences of the United States of America 115.8(2018).