气候变化领域集成服务门户(CCPortal): Cross-resistance to dicamba; 2;4-D; and fluroxypyr in Kochia scoparia is endowed by a mutation in an AUX/IAA gene

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DOI	10.1073/pnas.1712372115
	Cross-resistance to dicamba; 2;4-D; and fluroxypyr in Kochia scoparia is endowed by a mutation in an AUX/IAA gene
	LeClere S.; Wu C.; Westra P.; Sammons R.D.
发表日期	2018
ISSN	0027-8424
起始页码	E2911
结束页码	E2920
卷号	115 期号:13
英文摘要	The understanding and mitigation of the appearance of herbicide-resistant weeds have come to the forefront of study in the past decade, as the number of weed species that are resistant to one or more herbicide modes of action is on the increase. Historically, weed resistance to auxin herbicides has been rare, but examples, such as Kochia scoparia L. Schrad (kochia), have appeared, posing a challenge to conventional agricultural practices. Reports of dicamba-resistant kochia populations began in the early 1990s in areas where auxin herbicides were heavily utilized for weed control in corn and wheat cropping systems, and some biotypes are resistant to other auxin herbicides as well. We have further characterized the auxin responses of one previously reported dicamba-resistant biotype isolated from western Nebraska and found that it is additionally cross-resistant to other auxin herbicides, including 2,4-dichlorophenoxyacetic acid (2,4-D) and fluroxypyr. We have utilized transcriptome sequencing and comparison to identify a 2-nt base change in this biotype, which results in a glycine to asparagine amino acid change within a highly conserved region of an AUX/indole-3-acetic acid (IAA) protein, KsIAA16. Through yeast two-hybrid analysis, characterization of F2 segregation, and heterologous expression and characterization of the gene in Arabidopsis thaliana, we show that that the single dominant KsIAA16R resistance allele is the causal basis for dicamba resistance in this population. Furthermore, we report the development of a molecular marker to identify this allele in populations and facilitate inheritance studies. We also report that the resistance allele confers a fitness penalty in greenhouse studies. © 2018 National Academy of Sciences. All Rights Reserved.
英文关键词	Auxin; Herbicide; KsIAA16; KsTIR1; Weed resistance
语种	英语
scopus关键词	2,4 dichlorophenoxyacetic acid; asparagine; auxin; dicamba; fluroxypyr; glycine; herbicide; indoleacetic acid; molecular marker; unclassified drug; 2,4 dichlorophenoxyacetic acid; 4,5-amino-3,5-dichloro-6-fluoro-2-pyridinyloxyacetic acid; acetic acid; dicamba; herbicide; indoleacetic acid; indoleacetic acid derivative; plant protein; pyridine derivative; amino acid substitution; Arabidopsis thaliana; Article; AUX gene; biotype; controlled study; gene expression; gene function; gene mutation; gene segregation; gene sequence; genetic analysis; genetic resistance; genetic transcription; greenhouse; herbicide resistance; IAA gene; Kochia scoparia; KsIAA16 gene; Nebraska; nonhuman; nucleotide sequence; phenotype; plant gene; plant growth; plant leaf; priority journal; protein analysis; protein interaction; reporter gene; sequence analysis; wild type; Arabidopsis; drug effect; genetics; growth, development and aging; herbicide resistance; Kochia scoparia; mutation; physiology; weed; 2,4-Dichlorophenoxyacetic Acid; Acetates; Arabidopsis; Bassia scoparia; Dicamba; Herbicide Resistance; Herbicides; Indoleacetic Acids; Mutation; Plant Proteins; Plant Weeds; Pyridines
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160533
作者单位	LeClere, S., Agricultural Productivity Innovations Division, Monsanto, Chesterfield, MO 63017, United States; Wu, C., Agricultural Productivity Innovations Division, Monsanto, Chesterfield, MO 63017, United States; Westra, P., Department of Bioagricultural Sciences and Pest Management, College of Agricultural Sciences, Colorado State University, Fort Collins, CO 80523-1177, United States; Sammons, R.D., Agricultural Productivity Innovations Division, Monsanto, Chesterfield, MO 63017, United States
推荐引用方式 GB/T 7714	LeClere S.,Wu C.,Westra P.,et al. Cross-resistance to dicamba; 2;4-D; and fluroxypyr in Kochia scoparia is endowed by a mutation in an AUX/IAA gene[J],2018,115(13).
APA	LeClere S.,Wu C.,Westra P.,&Sammons R.D..(2018).Cross-resistance to dicamba; 2;4-D; and fluroxypyr in Kochia scoparia is endowed by a mutation in an AUX/IAA gene.Proceedings of the National Academy of Sciences of the United States of America,115(13).
MLA	LeClere S.,et al."Cross-resistance to dicamba; 2;4-D; and fluroxypyr in Kochia scoparia is endowed by a mutation in an AUX/IAA gene".Proceedings of the National Academy of Sciences of the United States of America 115.13(2018).