气候变化领域集成服务门户(CCPortal): Gibberellin DELLA signaling targets the retromer complex to redirect protein trafficking to the plasma membrane

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DOI	10.1073/pnas.1721760115
	Gibberellin DELLA signaling targets the retromer complex to redirect protein trafficking to the plasma membrane
	Salanenka Y.; Verstraeten I.; Löfke C.; Tabata K.; Naramoto S.; Glanc M.; Friml J.
发表日期	2018
ISSN	0027-8424
起始页码	3716
结束页码	3721
卷号	115 期号:14
英文摘要	The plant hormone gibberellic acid (GA) is a crucial regulator of growth and development. The main paradigm of GA signaling puts forward transcriptional regulation via the degradation of DELLA transcriptional repressors. GA has also been shown to regulate tropic responses by modulation of the plasma membrane incidence of PIN auxin transporters by an unclear mechanism. Here we uncovered the cellular and molecular mechanisms by which GA redirects protein trafficking and thus regulates cell surface functionality. Photoconver-tible reporters revealed that GA balances the protein traffic between the vacuole degradation route and recycling back to the cell surface. Low GA levels promote vacuolar delivery and degradation of multiple cargos, including PIN proteins, whereas high GA levels promote their recycling to the plasma membrane. This GA effect requires components of the retromer complex, such as Sorting Nexin 1 (SNX1) and its interacting, microtubule (MT)-associated protein, the Cytoplasmic Linker-Associated Protein (CLASP1). Accordingly, GA regulates the subcellular distribution of SNX1 and CLASP1, and the intact MT cytoskeleton is essential for the GA effect on trafficking. This GA cellular action occurs through DELLA proteins that regulate the MT and retromer presumably via their interaction partners Prefoldins (PFDs). Our study identified a branching of the GA signaling pathway at the level of DELLA proteins, which, in parallel to regulating transcription, also target by a nontranscriptional mechanism the retromer complex acting at the intersection of the degradation and recycling trafficking routes. By this mechanism, GA can redirect receptors and transporters to the cell surface, thus coregulat-ing multiple processes, including PIN-dependent auxin fluxes during tropic responses. © 2018 National Academy of Sciences. All Rights Reserved.
英文关键词	DELLA; Gibberellin; Microtubules; Polar auxin transport; Vesicle trafficking
语种	英语
scopus关键词	cytoplasmic linker associated protein 1; gibberellin; microtubule associated protein; prefoldin; protein DELLA; repressor protein; sorting nexin; sorting nexin 1; unclassified drug; Arabidopsis protein; GAI protein, Arabidopsis; gibberellin; indoleacetic acid derivative; phytohormone; SNX1 protein, Arabidopsis; sorting nexin; Article; cell membrane; cell migration; cell surface; controlled study; cytoskeleton; nonhuman; plant cell; priority journal; protein degradation; protein protein interaction; retromer complex; signal transduction; transcription regulation; Arabidopsis; cell membrane; cell vacuole; drug effect; gene expression regulation; genetics; growth, development and aging; metabolism; microtubule; protein transport; signal transduction; Arabidopsis; Arabidopsis Proteins; Cell Membrane; Gene Expression Regulation, Plant; Gibberellins; Indoleacetic Acids; Microtubules; Plant Growth Regulators; Protein Transport; Signal Transduction; Sorting Nexins; Vacuoles
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160528
作者单位	Salanenka, Y., Institute of Science and Technology Austria, Klosterneuburg, 3400, Austria; Verstraeten, I., Institute of Science and Technology Austria, Klosterneuburg, 3400, Austria; Löfke, C., Gregor Mendel Institute of Molecular Plant Biology, Vienna, 1030, Austria; Tabata, K., Institute of Science and Technology Austria, Klosterneuburg, 3400, Austria; Naramoto, S., Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980-8577, Japan; Glanc, M., Institute of Science and Technology Austria, Klosterneuburg, 3400, Austria, Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, 12844, Czech Republic; Friml, J., Institute of Science and Technology Austria, Klosterneuburg, 3400, Austria
推荐引用方式 GB/T 7714	Salanenka Y.,Verstraeten I.,Löfke C.,et al. Gibberellin DELLA signaling targets the retromer complex to redirect protein trafficking to the plasma membrane[J],2018,115(14).
APA	Salanenka Y..,Verstraeten I..,Löfke C..,Tabata K..,Naramoto S..,...&Friml J..(2018).Gibberellin DELLA signaling targets the retromer complex to redirect protein trafficking to the plasma membrane.Proceedings of the National Academy of Sciences of the United States of America,115(14).
MLA	Salanenka Y.,et al."Gibberellin DELLA signaling targets the retromer complex to redirect protein trafficking to the plasma membrane".Proceedings of the National Academy of Sciences of the United States of America 115.14(2018).