气候变化领域集成服务门户(CCPortal): Biomaterial surface energy-driven ligand assembly strongly regulates stem cell mechanosensitivity and fate on very soft substrates

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DOI	10.1073/pnas.1704543115
	Biomaterial surface energy-driven ligand assembly strongly regulates stem cell mechanosensitivity and fate on very soft substrates
	Razafiarison T.; Holenstein C.N.; Stauber T.; Jovic M.; Vertudes E.; Loparic M.; Kawecki M.; Bernard L.; Silvan U.; Snedeker J.G.
发表日期	2018
ISSN	0027-8424
起始页码	4631
结束页码	4636
卷号	115 期号:18
英文摘要	Although mechanisms of cell–material interaction and cellular mechanotransduction are increasingly understood, the mechanical insensitivity of mesenchymal cells to certain soft amorphous biomaterial substrates has remained largely unexplained. We reveal that surface energy-driven supramolecular ligand assembly can regulate mesenchymal stem cell (MSC) sensing of substrate mechanical compliance and subsequent cell fate. Human MSCs were cultured on collagen-coated hydrophobic polydimethylsiloxane (PDMS) and hydrophilic polyethylene-oxide-PDMS (PEO-PDMS) of a range of stiffnesses. Although cell contractility was similarly diminished on soft substrates of both types, cell spreading and osteogenic differentiation occurred only on soft PDMS and not hydrophilic PEO-PDMS (elastic modulus <1 kPa). Substrate surface energy yields distinct ligand topologies with accordingly distinct profiles of recruited transmembrane cell receptors and related focal adhesion signaling. These differences did not differentially regulate Rho-associated kinase activity, but nonetheless regulated both cell spreading and downstream differentiation. © 2018 National Academy of Sciences. All rights reserved.
英文关键词	Ligand assembly; Mechanobiology; PDMS; Stem cell; Surface energy
语种	英语
scopus关键词	biomaterial; collagen; dimeticone; macrogol; Rho kinase; biomaterial; Article; cell differentiation; cell fate; cell spreading; controlled study; enzyme activity; focal adhesion; human; human cell; hydrophilicity; hydrophobicity; mechanical stress; mesenchymal stem cell; priority journal; rigidity; stem cell culture; surface property; Young modulus; cell adhesion; cell proliferation; chemistry; cytology; mechanotransduction; mesenchymal stroma cell; metabolism; physiology; signal transduction; stem cell; surface tension; Biocompatible Materials; Cell Adhesion; Cell Differentiation; Cell Proliferation; Collagen; Elastic Modulus; Humans; Mechanotransduction, Cellular; Mesenchymal Stromal Cells; Signal Transduction; Stem Cells; Surface Tension
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160524
作者单位	Razafiarison, T., Department of Orthopedics, Balgrist University Hospital, University of Zurich, Zurich, 8008, Switzerland, Institute for Biomechanics, ETH Zurich, Zurich, 8008, Switzerland; Holenstein, C.N., Department of Orthopedics, Balgrist University Hospital, University of Zurich, Zurich, 8008, Switzerland, Institute for Biomechanics, ETH Zurich, Zurich, 8008, Switzerland; Stauber, T., Department of Orthopedics, Balgrist University Hospital, University of Zurich, Zurich, 8008, Switzerland, Institute for Biomechanics, ETH Zurich, Zurich, 8008, Switzerland; Jovic, M., Department of Orthopedics, Balgrist University Hospital, University of Zurich, Zurich, 8008, Switzerland, Institute for Biomechanics, ETH Zurich, Zurich, 8008, Switzerland; Vertudes, E., Department of Orthopedics, Balgrist University Hospital, University of Zurich, Zurich, 8008, Switzerland, Institute for Biomechanics, ETH Zurich, Zurich, 8008, Switzerland; Loparic, M., Biozentrum, Swiss Nanoscience Institute, University of Basel, Basel,...
推荐引用方式 GB/T 7714	Razafiarison T.,Holenstein C.N.,Stauber T.,et al. Biomaterial surface energy-driven ligand assembly strongly regulates stem cell mechanosensitivity and fate on very soft substrates[J],2018,115(18).
APA	Razafiarison T..,Holenstein C.N..,Stauber T..,Jovic M..,Vertudes E..,...&Snedeker J.G..(2018).Biomaterial surface energy-driven ligand assembly strongly regulates stem cell mechanosensitivity and fate on very soft substrates.Proceedings of the National Academy of Sciences of the United States of America,115(18).
MLA	Razafiarison T.,et al."Biomaterial surface energy-driven ligand assembly strongly regulates stem cell mechanosensitivity and fate on very soft substrates".Proceedings of the National Academy of Sciences of the United States of America 115.18(2018).