气候变化领域集成服务门户(CCPortal): Loss of a highly conserved sterile alpha motif domain gene (WEEP) results in pendulous branch growth in peach trees

CCPortal

DOI	10.1073/pnas.1704515115
	Loss of a highly conserved sterile alpha motif domain gene (WEEP) results in pendulous branch growth in peach trees
	Hollender C.A.; Pascal T.; Tabb A.; Hadiarto T.; Srinivasan C.; Wang W.; Liu Z.; Scorza R.; Dardick C.
发表日期	2018
ISSN	0027-8424
起始页码	E4690
结束页码	E4699
卷号	115 期号:20
英文摘要	Plant shoots typically grow upward in opposition to the pull of gravity. However, exceptions exist throughout the plant kingdom. Most conspicuous are trees with weeping or pendulous branches. While such trees have long been cultivated and appreciated for their ornamental value, the molecular basis behind the weeping habit is not known. Here, we characterized a weeping tree phenotype in Prunus persica (peach) and identified the underlying genetic mutation using a genomic sequencing approach. Weeping peach tree shoots exhibited a downward elliptical growth pattern and did not exhibit an upward bending in response to 90° reorientation. The causative allele was found to be an uncharacterized gene, Ppa013325, having a 1.8-Kb deletion spanning the 5′ end. This gene, dubbed WEEP, was predominantly expressed in phloem tissues and encodes a highly conserved 129-amino acid protein containing a sterile alpha motif (SAM) domain. Silencing WEEP in the related tree species Prunus domestica (plum) resulted in more outward, downward, and wandering shoot orientations compared to standard trees, supporting a role for WEEP in directing lateral shoot growth in trees. This previously unknown regulator of branch orientation, which may also be a regulator of gravity perception or response, provides insights into our understanding of how tree branches grow in opposition to gravity and could serve as a critical target for manipulating tree architecture for improved tree shape in agricultural and horticulture applications. © 2018 National Academy of Sciences. All rights reserved.
英文关键词	Gravitropism; Peach; SAM domain; Tree architecture; Weeping
语种	英语
scopus关键词	agricultural procedures; allele; Article; comparative study; controlled study; gene deletion; gene expression; gene mutation; gene sequence; gene silencing; gravity sensing; growth curve; horticulture; nonhuman; peach; phenotype; phloem; plant gene; plant tissue; priority journal; protein depletion; shoot; shoot growth; sterile alpha motif; tree growth; WEEP gene; anatomy and histology; chromosomal mapping; genetics; growth, development and aging; metabolism; phylogeny; plant root; protein domain; tree; plant protein; Chromosome Mapping; Phenotype; Phylogeny; Plant Proteins; Plant Roots; Plant Shoots; Protein Domains; Prunus persica; Sterile Alpha Motif; Trees
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160518
作者单位	Hollender, C.A., Appalachian Fruit Research Station, Agricultural Research Service, US Department of Agriculture, Kearneysville, WV 25430, United States, Department of Horticulture, College of Agriculture and Natural Resources, Michigan State University, East Lansing, MI 48824, United States; Pascal, T., Unité Génétique et Amélioration de Fruits et Légumes, Institut National de la Recherche Agronomique, Montfavet, 84140, France; Tabb, A., Appalachian Fruit Research Station, Agricultural Research Service, US Department of Agriculture, Kearneysville, WV 25430, United States; Hadiarto, T., Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (BB Biogen), Bogor, Indonesia; Srinivasan, C., Appalachian Fruit Research Station, Agricultural Research Service, US Department of Agriculture, Kearneysville, WV 25430, United States; Wang, W., Department of Cell Biology and Molecular Genetics, College of Mathematics and Natural Sciences, University of Maryland, College Park...
推荐引用方式 GB/T 7714	Hollender C.A.,Pascal T.,Tabb A.,et al. Loss of a highly conserved sterile alpha motif domain gene (WEEP) results in pendulous branch growth in peach trees[J],2018,115(20).
APA	Hollender C.A..,Pascal T..,Tabb A..,Hadiarto T..,Srinivasan C..,...&Dardick C..(2018).Loss of a highly conserved sterile alpha motif domain gene (WEEP) results in pendulous branch growth in peach trees.Proceedings of the National Academy of Sciences of the United States of America,115(20).
MLA	Hollender C.A.,et al."Loss of a highly conserved sterile alpha motif domain gene (WEEP) results in pendulous branch growth in peach trees".Proceedings of the National Academy of Sciences of the United States of America 115.20(2018).