气候变化领域集成服务门户(CCPortal): Imaging mycobacterial growth and division with a fluorogenic probe

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DOI	10.1073/pnas.1720996115
	Imaging mycobacterial growth and division with a fluorogenic probe
	Hodges H.L.; Brown R.A.; Crooks J.A.; Weibel D.B.; Kiessling L.L.
发表日期	2018
ISSN	0027-8424
起始页码	5271
结束页码	5276
卷号	115 期号:20
英文摘要	Control and manipulation of bacterial populations requires an understanding of the factors that govern growth, division, and antibiotic action. Fluorescent and chemically reactive small molecule probes of cell envelope components can visualize these processes and advance our knowledge of cell envelope biosynthesis (e.g., peptidoglycan production). Still, fundamental gaps remain in our understanding of the spatial and temporal dynamics of cell envelope assembly. Previously described reporters require steps that limit their use to static imaging. Probes that can be used for real-time imaging would advance our understanding of cell envelope construction. To this end, we synthesized a fluorogenic probe that enables continuous live cell imaging in mycobacteria and related genera. This probe reports on the mycolyltransferases that assemble the mycolic acid membrane. This peptidoglycan-anchored bilayer-like assembly functions to protect these cells from antibiotics and host defenses. Our probe, quencher-trehalose-fluorophore (QTF), is an analog of the natural mycolyltransferase substrate. Mycolyltransferases process QTF by diverting their normal transesterification activity to hydrolysis, a process that unleashes fluorescence. QTF enables high contrast continuous imaging and the visualization of mycolyltransferase activity in cells. QTF revealed that mycolyltransferase activity is augmented before cell division and localized to the septa and cell poles, especially at the old pole. This observed localization suggests that mycolyltransferases are components of extracellular cell envelope assemblies, in analogy to the intracellular divisomes and polar elongation complexes. We anticipate QTF can be exploited to detect and monitor mycobacteria in physiologically relevant environments. © 2018 National Academy of Sciences. All rights reserved.
英文关键词	Ag85; Cell wall; Lipid; Mycolic acid; Tuberculosis
语种	英语
scopus关键词	antibiotic agent; peptidoglycan; quencher trehalose fluorophore; transferase; unclassified drug; bacterial protein; cord factor; fluorescent dye; peptidoglycan; trehalose monomycolate; Article; bacterial growth; bacterial membrane; bacterial phenomena and functions; bacterium division; bilayer membrane; cell division; cellular distribution; complex formation; controlled study; enzyme activity; enzyme substrate; extracellular space; fluorescence analysis; host resistance; hydrolysis; image analysis; intracellular membrane; live cell imaging; molecular probe; Mycobacterium; nonhuman; priority journal; taxonomic rank; transesterification; cell wall; chemistry; Corynebacterium glutamicum; fluorescence; growth, development and aging; human; image processing; metabolism; microbiology; Mycobacterium tuberculosis; procedures; tuberculosis; Bacterial Proteins; Cell Division; Cell Wall; Cord Factors; Corynebacterium glutamicum; Fluorescence; Fluorescent Dyes; Humans; Image Processing, Computer-Assisted; Mycobacterium tuberculosis; Peptidoglycan; Tuberculosis
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160516
作者单位	Hodges, H.L., Department of Chemistry, University of Wisconsin–Madison, Madison, WI 53706, United States; Brown, R.A., Department of Chemistry, University of Wisconsin–Madison, Madison, WI 53706, United States; Crooks, J.A., Department of Biochemistry, University of Wisconsin–Madison, Madison, WI 53706, United States; Weibel, D.B., Department of Biochemistry, University of Wisconsin–Madison, Madison, WI 53706, United States; Kiessling, L.L., Department of Chemistry, University of Wisconsin–Madison, Madison, WI 53706, United States, Department of Biochemistry, University of Wisconsin–Madison, Madison, WI 53706, United States, Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, United States
推荐引用方式 GB/T 7714	Hodges H.L.,Brown R.A.,Crooks J.A.,et al. Imaging mycobacterial growth and division with a fluorogenic probe[J],2018,115(20).
APA	Hodges H.L.,Brown R.A.,Crooks J.A.,Weibel D.B.,&Kiessling L.L..(2018).Imaging mycobacterial growth and division with a fluorogenic probe.Proceedings of the National Academy of Sciences of the United States of America,115(20).
MLA	Hodges H.L.,et al."Imaging mycobacterial growth and division with a fluorogenic probe".Proceedings of the National Academy of Sciences of the United States of America 115.20(2018).