气候变化领域集成服务门户(CCPortal): Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein

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DOI	10.1073/pnas.1800635115
	Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein
	Mahatabuddin S.; Fukami D.; Arai T.; Nishimiya Y.; Shimizu R.; Shibazaki C.; Kondo H.; Adachi M.; Tsuda S.
发表日期	2018
ISSN	0027-8424
起始页码	5456
结束页码	5461
卷号	115 期号:21
英文摘要	Polypentagonal water networks were recently observed in a protein capable of binding to ice crystals, or ice-binding protein (IBP). To examine such water networks and clarify their role in ice-binding, we determined X-ray crystal structures of a 65-residue defective isoform of a Zoarcidae-derived IBP (wild type, WT) and its five single mutants (A20L, A20G, A20T, A20V, and A20I). Polypentagonal water networks composed of ∼50 semiclathrate waters were observed solely on the strongest A20I mutant, which appeared to include a tetrahedral water cluster exhibiting a perfect position match to the (1010) first prism plane of a single ice crystal. Inclusion of another symmetrical water cluster in the polypentagonal network showed a perfect complementarity to the waters constructing the (2021) pyramidal ice plane. The order of ice-binding strength was A20L < A20G < WT < A20T < A20V < A20I, where the top three mutants capable of binding to the first prism and the pyramidal ice planes commonly contained a bifurcated γ-CH3 group. These results suggest that a fine-tuning of the surface of Zoarcidae-derived IBP assisted by a side-chain group regulates the holding property of its polypentagonal water network, the function of which is to freeze the host protein to specific ice planes. © 2018 National Academy of Sciences. All rights reserved.
英文关键词	Crystal structure; Hydration shell; Ice-binding protein; Polypentagonal waters; Water cluster
语种	英语
scopus关键词	antifreeze protein; ice binding protein; unclassified drug; antifreeze protein; carrier protein; fish protein; ice; isoprotein; protein binding; water; Article; controlled study; enzyme activity; enzyme inhibition; hydrogen bond; information processing; Macrozoarces americanus; nonhuman; Perciformes; polymerase chain reaction; polypentagonal ice like water network; priority journal; protein expression; protein function; animal; binding site; biophysics; chemistry; fish; freezing; genetics; metabolism; mutation; protein conformation; X ray crystallography; Animals; Antifreeze Proteins; Binding Sites; Biophysical Phenomena; Carrier Proteins; Crystallography, X-Ray; Fish Proteins; Fishes; Freezing; Ice; Mutation; Protein Binding; Protein Conformation; Protein Isoforms; Water
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160513
作者单位	Mahatabuddin, S., Graduate School of Life Science, Hokkaido University, Sapporo, 060-0810, Japan; Fukami, D., Graduate School of Life Science, Hokkaido University, Sapporo, 060-0810, Japan; Arai, T., Graduate School of Life Science, Hokkaido University, Sapporo, 060-0810, Japan; Nishimiya, Y., Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, 062-8517, Japan; Shimizu, R., Quantum Beam Science Research Directorate, National Institute for Quantum and Radiological Science and Technology (QST), Ibaraki, 319-1106, Japan; Shibazaki, C., Quantum Beam Science Research Directorate, National Institute for Quantum and Radiological Science and Technology (QST), Ibaraki, 319-1106, Japan; Kondo, H., Graduate School of Life Science, Hokkaido University, Sapporo, 060-0810, Japan, Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, 062-8517, Japan; Adachi, M., Quantum Beam Science Research Dire...
推荐引用方式 GB/T 7714	Mahatabuddin S.,Fukami D.,Arai T.,et al. Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein[J],2018,115(21).
APA	Mahatabuddin S..,Fukami D..,Arai T..,Nishimiya Y..,Shimizu R..,...&Tsuda S..(2018).Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein.Proceedings of the National Academy of Sciences of the United States of America,115(21).
MLA	Mahatabuddin S.,et al."Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein".Proceedings of the National Academy of Sciences of the United States of America 115.21(2018).