气候变化领域集成服务门户(CCPortal): MIZ1 regulates ECA1 to generate a slow; long-distance phloem-transmitted Ca2+ signal essential for root water tracking in Arabidopsis

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DOI	10.1073/pnas.1804130115
	MIZ1 regulates ECA1 to generate a slow; long-distance phloem-transmitted Ca2+ signal essential for root water tracking in Arabidopsis
	Shkolnik D.; Nuriel R.; Bonza M.C.; Costa A.; Fromm H.
发表日期	2018
ISSN	0027-8424
起始页码	8031
结束页码	8036
卷号	115 期号:31
英文摘要	Ever since Darwin postulated that the tip of the root is sensitive to moisture differences and that it “transmits an influence to the upper adjoining part, which bends towards the source of moisture” [Darwin C, Darwin F (1880) The Power of Movement in Plants, pp 572–574], the signal underlying this tropic response has remained elusive. Using the FRET-based Cameleon Ca2+ sensor in planta, we show that a water potential gradient applied across the root tip generates a slow, long-distance asymmetric cytosolic Ca2+ signal in the phloem, which peaks at the elongation zone, where it is dispersed laterally and asymmetrically to peripheral cells, where cell elongation occurs. In addition, the MIZ1 protein, whose biochemical function is unknown but is required for root curvature toward water, is indispensable for generating the slow, long-distance Ca2+ signal. Furthermore, biochemical and genetic manipulations that elevate cytosolic Ca2+ levels, including mutants of the endoplasmic reticulum (ER) Ca2+-ATPase isoform ECA1, enhance root curvature toward water. Finally, coimmunoprecipitation of plant proteins and functional complementation assays in yeast cells revealed that MIZ1 directly binds to ECA1 and inhibits its activity. We suggest that the inhibition of ECA1 by MIZ1 changes the balance between cytosolic Ca2+ influx and efflux and generates the cytosolic Ca2+ signal required for water tracking. © 2018 National Academy of Sciences. All rights reserved.
英文关键词	Arabidopsis; Calcium; ECA1; Hydrotropism; MIZ1
语种	英语
scopus关键词	adenosine triphosphatase (calcium); MIZ1 protein; plant protein; unclassified drug; Arabidopsis protein; calcium; ECA1 protein, Arabidopsis; mizu-kussei1 protein, Arabidopsis; vesicular transport adaptor protein; water; Arabidopsis; Article; calcium signaling; calcium transport; cell elongation; chromatin immunoprecipitation; controlled study; enzyme regulation; genetic manipulation; nonhuman; phytochemistry; plant root; plant water potential; priority journal; protein binding; yeast cell; Arabidopsis; calcium signaling; cytosol; metabolism; phloem; physiology; plant root; Adaptor Proteins, Vesicular Transport; Arabidopsis; Arabidopsis Proteins; Calcium; Calcium Signaling; Cytosol; Phloem; Plant Roots; Water
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160486
作者单位	Shkolnik, D., School of Plant Sciences and Food Security, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, 69978, Israel; Nuriel, R., School of Plant Sciences and Food Security, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, 69978, Israel; Bonza, M.C., Department of Biosciences, University of Milan, Milan, 20133, Italy; Costa, A., Department of Biosciences, University of Milan, Milan, 20133, Italy, Institute of Biophysics, Consiglio Nazionale delle Ricerche, Milan, 20133, Italy; Fromm, H., School of Plant Sciences and Food Security, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, 69978, Israel
推荐引用方式 GB/T 7714	Shkolnik D.,Nuriel R.,Bonza M.C.,et al. MIZ1 regulates ECA1 to generate a slow; long-distance phloem-transmitted Ca2+ signal essential for root water tracking in Arabidopsis[J],2018,115(31).
APA	Shkolnik D.,Nuriel R.,Bonza M.C.,Costa A.,&Fromm H..(2018).MIZ1 regulates ECA1 to generate a slow; long-distance phloem-transmitted Ca2+ signal essential for root water tracking in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America,115(31).
MLA	Shkolnik D.,et al."MIZ1 regulates ECA1 to generate a slow; long-distance phloem-transmitted Ca2+ signal essential for root water tracking in Arabidopsis".Proceedings of the National Academy of Sciences of the United States of America 115.31(2018).