气候变化领域集成服务门户(CCPortal): Ladderane phospholipids form a densely packed membrane with normal hydrazine and anomalously low proton/hydroxide permeability

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DOI	10.1073/pnas.1810706115
	Ladderane phospholipids form a densely packed membrane with normal hydrazine and anomalously low proton/hydroxide permeability
	Moss F.R.; Shuken S.R.; Mercer J.A.M.; Cohen C.M.; Weiss T.M.; Boxer S.G.; Burns N.Z.
发表日期	2018
ISSN	0027-8424
起始页码	9098
结束页码	9103
卷号	115 期号:37
英文摘要	Ladderane lipids are unique to anaerobic ammonium-oxidizing (anammox) bacteria and are enriched in the membrane of the anammoxosome, an organelle thought to compartmentalize the anammox process, which involves the toxic intermediate hydrazine (N2H4). Due to the slow growth rate of anammox bacteria and difficulty of isolating pure ladderane lipids, experimental evidence of the biological function of ladderanes is lacking. We have synthesized two natural and one unnatural ladderane phosphatidylcholine lipids and compared their thermotropic properties in self-assembled bilayers to distinguish between [3]- and [5]-ladderane function. We developed a hydrazine transmembrane diffusion assay using a water-soluble derivative of a hydrazine sensor and determined that ladderane membranes are as permeable to hydrazine as straight-chain lipid bilayers. However, pH equilibration across ladderane membranes occurs 5–10 times more slowly than across straight-chain lipid membranes. Langmuir monolayer analysis and the rates of fluorescence recovery after photobleaching suggest that dense ladderane packing may preclude formation of proton/hydroxide-conducting water wires. These data support the hypothesis that ladderanes prevent the breakdown of the proton motive force rather than blocking hydrazine transmembrane diffusion in anammox bacteria. © 2018 National Academy of Sciences. All Rights Reserved.
英文关键词	Anammox; Ladderane; Lipid bilayer; Membrane structure; Proton permeability
语种	英语
scopus关键词	hydrazine; hydroxide; Ladderane phospholipid; phosphatidylcholine; phospholipid derivative; proton; unclassified drug; hydrazine derivative; hydroxide; phospholipid; anammox bacterium; Article; bioassay; bleaching; controlled study; fluorescence; lipid analysis; lipid bilayer; membrane permeability; nonhuman; pH; priority journal; solubility; synthesis; anaerobic growth; bacterium; cell membrane; cell membrane permeability; chemistry; metabolism; physiology; Anaerobiosis; Bacteria; Cell Membrane; Cell Membrane Permeability; Hydrazines; Hydroxides; Phospholipids; Protons
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160465
作者单位	Moss, F.R., Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Shuken, S.R., Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Mercer, J.A.M., Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Cohen, C.M., Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Weiss, T.M., Stanford Synchrotron Radiation Laboratory, Stanford Linear Accelerator Center, Stanford University, Menlo Park, CA 94025, United States; Boxer, S.G., Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Burns, N.Z., Department of Chemistry, Stanford University, Stanford, CA 94305, United States
推荐引用方式 GB/T 7714	Moss F.R.,Shuken S.R.,Mercer J.A.M.,et al. Ladderane phospholipids form a densely packed membrane with normal hydrazine and anomalously low proton/hydroxide permeability[J],2018,115(37).
APA	Moss F.R..,Shuken S.R..,Mercer J.A.M..,Cohen C.M..,Weiss T.M..,...&Burns N.Z..(2018).Ladderane phospholipids form a densely packed membrane with normal hydrazine and anomalously low proton/hydroxide permeability.Proceedings of the National Academy of Sciences of the United States of America,115(37).
MLA	Moss F.R.,et al."Ladderane phospholipids form a densely packed membrane with normal hydrazine and anomalously low proton/hydroxide permeability".Proceedings of the National Academy of Sciences of the United States of America 115.37(2018).