气候变化领域集成服务门户(CCPortal): Extensile motor activity drives coherent motions in a model of interphase chromatin

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DOI	10.1073/pnas.1807073115
	Extensile motor activity drives coherent motions in a model of interphase chromatin
	Saintillan D.; Shelley M.J.; Zidovska A.
发表日期	2018
ISSN	0027-8424
起始页码	11442
结束页码	11447
卷号	115 期号:45
英文摘要	The 3D spatiotemporal organization of the human genome inside the cell nucleus remains a major open question in cellular biology. In the time between two cell divisions, chromatin-the functional form of DNA in cells-fills the nucleus in its uncon-densed polymeric form. Recent in vivo imaging experiments reveal that the chromatin moves coherently, having displacements with long-ranged correlations on the scale of micrometers and lasting for seconds. To elucidate the mechanism(s) behind these motions, we develop a coarse-grained active polymer model where chromatin is represented as a confined flexible chain acted upon by molecular motors that drive fluid flows by exerting dipolar forces on the system. Numerical simulations of this model account for steric and hydrodynamic interactions as well as internal chain mechanics. These demonstrate that coherent motions emerge in systems involving extensile dipoles and are accompanied by large-scale chain reconfigurations and nematic ordering. Comparisons with experiments show good qualitative agreement and support the hypothesis that self-organizing long-ranged hydrodynamic couplings between chromatin-associated active motor proteins are responsible for the observed coherent dynamics. © 2018 National Academy of Sciences. All rights reserved.
英文关键词	Active matter; Chromatin; Nucleoplasm; Polymer dynamics
语种	英语
scopus关键词	molecular motor; adenosine triphosphate; green fluorescent protein; histone; Article; cell nucleus; chromatin; chromatin structure; chromosomes and chromosomal phenomena; coherent motion; conformation; correlational study; dipole; fluid flow; hydrodynamics; interphase; motor activity; priority journal; qualitative analysis; simulation; structure analysis; wild type; chromatin; gene expression; genetics; HeLa cell line; human; liquid crystal; metabolism; molecular model; ultrastructure; Adenosine Triphosphate; Chromatin; Gene Expression; Green Fluorescent Proteins; HeLa Cells; Histones; Humans; Interphase; Liquid Crystals; Models, Molecular; Molecular Motor Proteins
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160448
作者单位	Saintillan, D., Department of Mechanical and Aerospace Engineering, University of California, San Diego, San Diego, CA 92093, United States; Shelley, M.J., Center for Computational Biology, Flatiron Institute, New York, NY 10010, United States, Courant Institute, New York University, New York, NY 10012, United States; Zidovska, A., Center for Soft Matter Research, Department of Physics, New York University, New York, NY 10003, United States
推荐引用方式 GB/T 7714	Saintillan D.,Shelley M.J.,Zidovska A.. Extensile motor activity drives coherent motions in a model of interphase chromatin[J],2018,115(45).
APA	Saintillan D.,Shelley M.J.,&Zidovska A..(2018).Extensile motor activity drives coherent motions in a model of interphase chromatin.Proceedings of the National Academy of Sciences of the United States of America,115(45).
MLA	Saintillan D.,et al."Extensile motor activity drives coherent motions in a model of interphase chromatin".Proceedings of the National Academy of Sciences of the United States of America 115.45(2018).