气候变化领域集成服务门户(CCPortal): Golgi-localized LOT regulates trans-Golgi network biogenesis and pollen tube growth

CCPortal

DOI	10.1073/pnas.1809206115
	Golgi-localized LOT regulates trans-Golgi network biogenesis and pollen tube growth
	Jia P.-F.; Xue Y.; Li H.-J.; Yang W.-C.
发表日期	2018
ISSN	0027-8424
起始页码	12307
结束页码	12312
卷号	115 期号:48
英文摘要	The trans-Golgi network (TGN) is an essential tubular-vesicular organelle derived from the Golgi and functions as an independent sorting and trafficking hub within the cell. However, the molecular regulation of TGN biogenesis remains enigmatic. Here we identified an Arabidopsis mutant loss of TGN (lot) that is defective in TGN formation and sterile due to impaired pollen tube growth in the style. The mutation leads to overstacking of the Golgi cisternae and significant reduction in the number of TGNs and vesicles surrounding the Golgi in pollen, which is corroborated by the dispersed cytosolic distribution of TGN-localized proteins. Consistently, deposition of extracellular pectin and plasma membrane localization of kinases and phosphoinositide species are also impaired. Subcellular localization analysis suggests that LOT is localized on the periphery of the Golgi cisternae, but the mutation does not affect the localization of Golgi-resident proteins. Furthermore, the yeast complementation result suggests that LOT could functionally act as a component of the guanine nucleotide exchange factor (GEF) complex of small Rab GTPase Ypt6. Taken together, these findings suggest that LOT is a critical player for TGN biogenesis in the plant lineage. © 2018 National Academy of Sciences. All Rights Reserved.
英文关键词	Golgi; Pollen tube growth; TGN biogenesis; Vesicle trafficking
语种	英语
scopus关键词	Arabidopsis protein; guanine nucleotide exchange factor; loss of TGN protein; pectin; phosphatidylinositide; phosphotransferase; Rab protein; unclassified drug; Ypt6 protein; Arabidopsis protein; guanine nucleotide exchange factor; Arabidopsis; Article; biogenesis; cell count; cell membrane; cell vacuole; cellular distribution; controlled study; cytosol; enzyme localization; extracellular matrix; gene mutation; lot gene; nonhuman; plant gene; pollen tube growth; priority journal; protein localization; regulatory mechanism; trans Golgi network; yeast; genetics; Golgi complex; growth, development and aging; metabolism; pollen tube; protein transport; trans Golgi network; Arabidopsis; Arabidopsis Proteins; Golgi Apparatus; Guanine Nucleotide Exchange Factors; Pollen Tube; Protein Transport; trans-Golgi Network
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160443
作者单位	Jia, P.-F., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China, University of Chinese Academy of Sciences, Beijing, 100049, China; Xue, Y., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China, University of Chinese Academy of Sciences, Beijing, 100049, China; Li, H.-J., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China, University of Chinese Academy of Sciences, Beijing, 100049, China; Yang, W.-C., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China, University of Chinese Academy of Sciences, Beijing, 100049, China
推荐引用方式 GB/T 7714	Jia P.-F.,Xue Y.,Li H.-J.,et al. Golgi-localized LOT regulates trans-Golgi network biogenesis and pollen tube growth[J],2018,115(48).
APA	Jia P.-F.,Xue Y.,Li H.-J.,&Yang W.-C..(2018).Golgi-localized LOT regulates trans-Golgi network biogenesis and pollen tube growth.Proceedings of the National Academy of Sciences of the United States of America,115(48).
MLA	Jia P.-F.,et al."Golgi-localized LOT regulates trans-Golgi network biogenesis and pollen tube growth".Proceedings of the National Academy of Sciences of the United States of America 115.48(2018).