气候变化领域集成服务门户(CCPortal): Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity

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DOI	10.1073/pnas.1813058115
	Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity
	Wang Q.; Li Y.; Ishikawa K.; Kosami K.-I.; Uno K.; Nagawa S.; Tan L.; Du J.; Shimamoto K.; Kawano Y.
发表日期	2018
ISSN	0027-8424
起始页码	E11551
结束页码	E11560
卷号	115 期号:49
英文摘要	Resistance (R) genes encode intracellular nucleotide-binding/ leucine-rich repeat-containing (NLR) family proteins that serve as critical plant immune receptors to induce effector-triggered immunity (ETI). NLR proteins possess a tripartite domain architecture consisting of an N-terminal variable region, a central nucleotide-binding domain, and a C-terminal leucine-rich repeat. N-terminal coiled-coil (CC) or Toll-interleukin 1 receptor (TIR) domains of R proteins appear to serve as platforms to trigger immune responses, because overexpression of the CC or TIR domain of some R proteins is sufficient to induce an immune response. Because direct downstream signaling molecules of R proteins remain obscure, the molecular mechanisms by which R proteins regulate downstream signaling are largely unknown. We reported previously that a rice R protein named Pit triggers ETI through a small GTPase, OsRac1, although how Pit activates OsRac1 is unclear. Here, we identified OsSPK1, a DOCK family guanine nucleotide exchange factor, as an interactor of Pit and activator for OsRac1. OsSPK1 contributes to signaling by two disease-resistance genes, Pit and Pia, against the rice blast fungus Magnaporthe oryzae and facilitates OsRac1 activation in vitro and in vivo. The CC domain of Pit is required for its binding to OsSPK1, OsRac1 activation, and the induction of cell death. Overall, we conclude that OsSPK1 is a direct and key signaling target of Pit-mediated immunity. Our results shed light on how R proteins trigger ETI through direct downstream molecules. © 2018 National Academy of Sciences. All rights reserved.
英文关键词	Effector-triggered immunity; GEF; R protein; Rice; Small GTPase
语种	英语
scopus关键词	guanine nucleotide exchange factor; Pit protein; plant protein; Rac1 protein; SPK1 protein; unclassified drug; plant protein; Article; cell death; controlled study; in vitro study; in vivo study; Magnaporthe oryzae; nonhuman; Pia gene; Pit gene; plant gene; plant genome; plant immunity; priority journal; protein analysis; protein binding; protein domain; protein function; protein interaction; rice; signal transduction; gene expression regulation; genetics; immunology; Magnaporthe; metabolism; microbiology; Oryza; plant disease; Gene Expression Regulation, Plant; Magnaporthe; Oryza; Plant Diseases; Plant Proteins
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160442
作者单位	Wang, Q., Signal Transduction and Immunity Group, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai, 201602, China, University of Chinese Academy of Sciences, Beijing, 100049, China; Li, Y., Signal Transduction and Immunity Group, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai, 201602, China, University of Chinese Academy of Sciences, Beijing, 100049, China; Ishikawa, K., Signal Transduction and Immunity Group, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai, 201602, China, Department of Genomics and Breeding, Iwate Biotechnology Research Center, Iwate, 024-0003, Japan; Kosami, K.-I., Signal Transduction and Immunity Group, Chinese Academy of Sciences Center for Excellence in Mol...
推荐引用方式 GB/T 7714	Wang Q.,Li Y.,Ishikawa K.,et al. Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity[J],2018,115(49).
APA	Wang Q..,Li Y..,Ishikawa K..,Kosami K.-I..,Uno K..,...&Kawano Y..(2018).Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity.Proceedings of the National Academy of Sciences of the United States of America,115(49).
MLA	Wang Q.,et al."Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity".Proceedings of the National Academy of Sciences of the United States of America 115.49(2018).