气候变化领域集成服务门户(CCPortal): Shigella promotes major alteration of gut epithelial physiology and tissue invasion by shutting off host intracellular transport

CCPortal

DOI	10.1073/pnas.1902922116
	Shigella promotes major alteration of gut epithelial physiology and tissue invasion by shutting off host intracellular transport
	Ferrari M.L.; Malardé V.; Grassart A.; Salavessa L.; Nigro G.; Descorps-Declere S.; Rohde J.R.; Schnupf P.; Masson V.; Arras G.; Loew D.; Sansonetti P.J.; Sauvonnet N.
发表日期	2019
ISSN	0027-8424
起始页码	13582
结束页码	13591
卷号	116 期号:27
英文摘要	Intracellular trafficking pathways in eukaryotic cells are essential to maintain organelle identity and structure, and to regulate cell communication with its environment. Shigella flexneri invades and subverts the human colonic epithelium by the injection of virulence factors through a type 3 secretion system (T3SS). In this work, we report the multiple effects of two S. flexneri effectors, IpaJ and VirA, which target small GTPases of the Arf and Rab families, consequently inhibiting several intracellular trafficking pathways. IpaJ and VirA induce large-scale impairment of host protein secretion and block the recycling of surface receptors. Moreover, these two effectors decrease clathrin-dependent and -independent endocytosis. Therefore, S. flexneri infection induces a global blockage of host cell intracellular transport, affecting the exchange between cells and their external environment. The combined action of these effectors disorganizes the epithelial cell polarity, disturbs epithelial barrier integrity, promotes multiple invasion events, and enhances the pathogen capacity to penetrate into the colonic tissue in vivo. © 2019 National Academy of Sciences. All rights reserved.
英文关键词	Bacteria; Endocytosis; Pathogen; Polarity; Secretion
语种	英语
scopus关键词	bacterial protein; protein IpaJ; protein VirA; unclassified drug; animal experiment; animal model; animal tissue; Article; bacterial growth; bacterial strain; bioinformatics; controlled study; endocytosis; flow cytometry; fluorescence microscopy; Golgi complex; human; human cell; immunomodulation; in vitro study; in vivo study; intestine epithelium; intracellular transport; liquid chromatography-mass spectrometry; nonhuman; pathogenesis; phenotype; plasmid; priority journal; protein secretion; protein targeting; protein transport; Shigella flexneri; shigellosis; species invasion; total internal reflection fluorescence microscopy
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160424
作者单位	Ferrari, M.L., Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, 75015, France, INSERM U1202, Paris, 75015, France; Malardé, V., Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, 75015, France, INSERM U1202, Paris, 75015, France; Grassart, A., Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, 75015, France, INSERM U1202, Paris, 75015, France; Salavessa, L., Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, 75015, France, INSERM U1202, Paris, 75015, France, Université Paris Sud, Paris-Saclay University, Orsay, 91400, France; Nigro, G., Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, 75015, France, INSERM U1202, Paris, 75015, France; Descorps-Declere, S., Institut Pasteur–Hub Bioinformatique et Biostatistique–Centre de Bioinformatique, Biostatistique et Biologie Intégrative, Unité de Services et de Recherche 3756 IP CNRS, Paris, 75015, France; Rohde, J.R., Department of Microbiology and Immunology, Dalhousi...
推荐引用方式 GB/T 7714	Ferrari M.L.,Malardé V.,Grassart A.,et al. Shigella promotes major alteration of gut epithelial physiology and tissue invasion by shutting off host intracellular transport[J],2019,116(27).
APA	Ferrari M.L..,Malardé V..,Grassart A..,Salavessa L..,Nigro G..,...&Sauvonnet N..(2019).Shigella promotes major alteration of gut epithelial physiology and tissue invasion by shutting off host intracellular transport.Proceedings of the National Academy of Sciences of the United States of America,116(27).
MLA	Ferrari M.L.,et al."Shigella promotes major alteration of gut epithelial physiology and tissue invasion by shutting off host intracellular transport".Proceedings of the National Academy of Sciences of the United States of America 116.27(2019).