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DOI	10.1073/pnas.1820455116
	Systematic mapping of cell wall mechanics in the regulation of cell morphogenesis
	Davì V.; Chevalier L.; Guo H.; Tanimoto H.; Barrett K.; Couturier E.; Boudaoud A.; Minc N.
发表日期	2019
ISSN	0027-8424
起始页码	13833
结束页码	13838
卷号	116 期号:28
英文摘要	Walled cells of plants, fungi, and bacteria come with a large range of shapes and sizes, which are ultimately dictated by the mechanics of their cell wall. This stiff and thin polymeric layer encases the plasma membrane and protects the cells mechanically by opposing large turgor pressure derived mechanical stresses. To date, however, we still lack a quantitative understanding for how local and/or global mechanical properties of the wall support cell morphogenesis. Here, we combine subresolution imaging and laser-mediated wall relaxation to quantitate subcellular values of wall thickness (h) and bulk elastic moduli (Y) in large populations of live mutant cells and in conditions affecting cell diameter in the rod-shaped model fission yeast. We find that lateral wall stiffness, defined by the surface modulus, σ = hY, robustly scales with cell diameter. This scaling is valid across tens of mutants spanning various functions—within the population of individual isogenic strains, along single misshaped cells, and even across the fission yeasts clade. Dynamic modulations of cell diameter by chemical and/or mechanical means suggest that the cell wall can rapidly adapt its surface mechanics, rendering stretched wall portions stiffer than unstretched ones. Size-dependent wall stiffening constrains diameter definition and limits size variations; it may also provide an efficient means to keep elastic strains in the wall below failure strains, potentially promoting cell survival. This quantitative set of data impacts our current understanding of the mechanics of cell walls and its contribution to morphogenesis. © 2019 National Academy of Sciences. All rights reserved.
英文关键词	Cell shape; Cell surface mechanics; Cell wall; Fission yeast
语种	英语
scopus关键词	Article; cell maturation; cell size; cell surface; cell survival; cell viability; cell wall; cellular parameters; cladistics; controlled study; fungal strain; imaging; nonhuman; priority journal; quantitative analysis; Schizosaccharomyces pombe; biomechanics; cell membrane; cell wall; chemistry; growth, development and aging; mechanical stress; morphogenesis; pressure; Schizosaccharomyces; surface property; Young modulus; actin; Actins; Biomechanical Phenomena; Cell Membrane; Cell Wall; Elastic Modulus; Morphogenesis; Pressure; Schizosaccharomyces; Stress, Mechanical; Surface Properties
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160397
作者单位	Davì, V., Institut Jacques Monod, CNRS, Université de Paris, Paris Cedex 13, 75205, France; Chevalier, L., Institut Jacques Monod, CNRS, Université de Paris, Paris Cedex 13, 75205, France; Guo, H., Institut Jacques Monod, CNRS, Université de Paris, Paris Cedex 13, 75205, France, Center for Research and Interdisciplinarity, Université de Paris, Paris, 75004, France; Tanimoto, H., Institut Jacques Monod, CNRS, Université de Paris, Paris Cedex 13, 75205, France, Department of Materials Science, Yokohama City University, Yokohama, 236-0004, Japan; Barrett, K., Institut Jacques Monod, CNRS, Université de Paris, Paris Cedex 13, 75205, France; Couturier, E., Laboratoire Matières et Systèmes Complexes, UMR 7057, CNRS, Université de Paris, Paris Cedex 13, 75205, France; Boudaoud, A., Reproduction et Développement des Plantes, École Normale Supérieure de Lyon, Claude Bernard Lyon I, Inra, Université de Lyon, CNRS, Lyon Cedex 07, 69364, France; Minc, N., Institut Jacques Monod, CNRS, Université de Paris, Paris Cedex...
推荐引用方式 GB/T 7714	Davì V.,Chevalier L.,Guo H.,et al. Systematic mapping of cell wall mechanics in the regulation of cell morphogenesis[J],2019,116(28).
APA	Davì V..,Chevalier L..,Guo H..,Tanimoto H..,Barrett K..,...&Minc N..(2019).Systematic mapping of cell wall mechanics in the regulation of cell morphogenesis.Proceedings of the National Academy of Sciences of the United States of America,116(28).
MLA	Davì V.,et al."Systematic mapping of cell wall mechanics in the regulation of cell morphogenesis".Proceedings of the National Academy of Sciences of the United States of America 116.28(2019).