气候变化领域集成服务门户(CCPortal): Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties

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DOI	10.1073/pnas.1821038116
	Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties
	Boeynaems S.; Holehouse A.S.; Weinhardt V.; Kovacs D.; Van Lindt J.; Larabell C.; Bosch L.V.D.; Das R.; Tompa P.S.; Pappu R.V.; Gitler A.D.
发表日期	2019
ISSN	0027-8424
起始页码	7889
结束页码	7898
卷号	116 期号:16
英文摘要	Phase separation of multivalent protein and RNA molecules underlies the biogenesis of biomolecular condensates such as membraneless organelles. In vivo, these condensates encompass hundreds of distinct types of molecules that typically organize into multilayered structures supporting the differential partitioning of molecules into distinct regions with distinct material properties. The interplay between driven (active) versus spontaneous (passive) processes that are required for enabling the formation of condensates with coexisting layers of distinct material properties remains unclear. Here, we deploy systematic experiments and simulations based on coarse-grained models to show that the collective interactions among the simplest, biologically relevant proteins and archetypal RNA molecules are sufficient for driving the spontaneous emergence of multilayered condensates with distinct material properties. These studies yield a set of rules regarding homotypic and heterotypic interactions that are likely to be relevant for understanding the interplay between active and passive processes that control the formation of functional biomolecular condensates. © 2019 National Academy of Sciences. All rights reserved.
英文关键词	Biomolecular condensates; Complex coacervation; Intrinsically disordered proteins; Phase transitions; RNA
语种	英语
scopus关键词	archetypal RNA; peptide derivative; RNA; unclassified drug; intrinsically disordered protein; RNA; Article; experimental study; molecular dynamics; molecular genetics; molecular model; priority journal; protein RNA binding; RNA sequence; RNA structure; simulation; biology; cell organelle; chemistry; metabolism; molecular dynamics; phase transition; physiology; Computational Biology; Intrinsically Disordered Proteins; Molecular Dynamics Simulation; Organelles; Phase Transition; RNA
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160370
作者单位	Boeynaems, S., Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, United States; Holehouse, A.S., Department of Biomedical Engineering, Washington University, St. Louis, MO 63130, United States, Center for Science and Engineering of Living Systems, Washington University, St. Louis, MO 63130, United States; Weinhardt, V., Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States, Department of Anatomy, University of California, San Francisco, CA 94143, United States; Kovacs, D., Center for Structural Biology, Vlaams Instituut voor Biotechnologie, Vrije Universiteit Brussel, Brussels, B-1050, Belgium; Van Lindt, J., Center for Structural Biology, Vlaams Instituut voor Biotechnologie, Vrije Universiteit Brussel, Brussels, B-1050, Belgium; Larabell, C., Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States, Department of Anat...
推荐引用方式 GB/T 7714	Boeynaems S.,Holehouse A.S.,Weinhardt V.,et al. Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties[J],2019,116(16).
APA	Boeynaems S..,Holehouse A.S..,Weinhardt V..,Kovacs D..,Van Lindt J..,...&Gitler A.D..(2019).Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties.Proceedings of the National Academy of Sciences of the United States of America,116(16).
MLA	Boeynaems S.,et al."Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties".Proceedings of the National Academy of Sciences of the United States of America 116.16(2019).