气候变化领域集成服务门户(CCPortal): Asymmetric division yields progeny cells with distinct modes of regulating cell cycle-dependent chromosome methylation

CCPortal

DOI	10.1073/pnas.1906119116
	Asymmetric division yields progeny cells with distinct modes of regulating cell cycle-dependent chromosome methylation
	Zhou X.; Wang J.; Herrmann J.; Moerner W.E.; Shapiro L.
发表日期	2019
ISSN	0027-8424
起始页码	15661
结束页码	15670
卷号	116 期号:31
英文摘要	The cell cycle-regulated methylation state of Caulobacter DNA mediates the temporal control of transcriptional activation of several key regulatory proteins. Temporally controlled synthesis of the CcrM DNA methyltransferase and Lon-mediated proteolysis restrict CcrM to a specific time in the cell cycle, thereby allowing the maintenance of the hemimethylated state of the chromosome during the progression of DNA replication. We determined that a chromosomal DNA-based platform stimulates CcrM degradation by Lon and that the CcrM C terminus both binds to its DNA substrate and is recognized by the Lon protease. Upon asymmetric cell division, swarmer and stalked progeny cells employ distinct mechanisms to control active CcrM. In progeny swarmer cells, CcrM is completely degraded by Lon before its differentiation into a replication-competent stalked cell later in the cell cycle. In progeny stalked cells, however, accumulated CcrM that has not been degraded before the immediate initiation of DNA replication is sequestered to the cell pole. Single-molecule imaging demonstrated physical anticorrelation between sequestered CcrM and chromosomal DNA, thus preventing DNA remethylation. The distinct control of available CcrM in progeny swarmer and stalked cells serves to protect the hemimethylated state of DNA during chromosome replication, enabling robustness of cell cycle progression. © 2019 National Academy of Sciences. All rights reserved.
英文关键词	DNA methyltransferase; Epigenetics; Lon protease; Protein sequestration
语种	英语
scopus关键词	bacterial DNA; DNA methyltransferase; endopeptidase La; bacterial DNA; bacterial protein; Article; asymmetric cell division; bacterial cell; bacterial chromosome; Caulobacter vibrioides; cell cycle progression; cell cycle regulation; cell differentiation; controlled study; DNA methylation; DNA replication; enzyme degradation; nonhuman; priority journal; progeny; protein degradation; single molecule imaging; transcription initiation; bacterial chromosome; biosynthesis; Caulobacter; cell cycle; DNA replication; genetics; metabolism; Bacterial Proteins; Caulobacter; Cell Cycle; Chromosomes, Bacterial; DNA Methylation; DNA Replication; DNA, Bacterial
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160359
作者单位	Zhou, X., Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, United States; Wang, J., Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Herrmann, J., Department of Structural Biology, Stanford University, Stanford, CA 94305, United States; Moerner, W.E., Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Shapiro, L., Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, United States, Chan Zuckerberg Biohub, San Francisco, CA 94158, United States
推荐引用方式 GB/T 7714	Zhou X.,Wang J.,Herrmann J.,et al. Asymmetric division yields progeny cells with distinct modes of regulating cell cycle-dependent chromosome methylation[J],2019,116(31).
APA	Zhou X.,Wang J.,Herrmann J.,Moerner W.E.,&Shapiro L..(2019).Asymmetric division yields progeny cells with distinct modes of regulating cell cycle-dependent chromosome methylation.Proceedings of the National Academy of Sciences of the United States of America,116(31).
MLA	Zhou X.,et al."Asymmetric division yields progeny cells with distinct modes of regulating cell cycle-dependent chromosome methylation".Proceedings of the National Academy of Sciences of the United States of America 116.31(2019).