气候变化领域集成服务门户(CCPortal): Mitotic and pheromone-specific intrinsic polarization cues interfere with gradient sensing in Saccharomyces cerevisiae

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DOI	10.1073/pnas.1912505117
	Mitotic and pheromone-specific intrinsic polarization cues interfere with gradient sensing in Saccharomyces cerevisiae
	Vasen G.; Dunayevich P.; Colman-Lerner A.
发表日期	2020
ISSN	0027-8424
起始页码	6580
结束页码	6589
卷号	117 期号:12
英文摘要	Polarity decisions are central to many processes, including mitosis and chemotropism. In Saccharomyces cerevisiae, budding and mating projection (MP) formation use an overlapping system of cortical landmarks that converges on the small G protein Cdc42. However, pheromone-gradient sensing must override the Rsr1-dependent internal polarity cues used for budding. Using this model system, we asked what happens when intrinsic and extrinsic spatial cues are not aligned. Is there competition, or collaboration? By live-cell microscopy and microfluidics techniques, we uncovered three previously overlooked features of this signaling system. First, the cytokinesis-associated polarization patch serves as a polarity landmark independently of all known cues. Second, the Rax1-Rax2 complex functions as a pheromone-promoted polarity cue in the distal pole of the cells. Third, internal cues remain active during pheromone-gradient tracking and can interfere with this process, biasing the location of MPs. Yeast defective in internal-cue utilization align significantly better than wild type with artificially generated pheromone gradients. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Chemotropism; Cytokinesis; Rax1; Rsr1; Signal integration
语种	英语
scopus关键词	fungal protein; pheromone; Rax1 protein; Rax2 protein; unclassified drug; mating factor; membrane protein; protein Cdc42; Rab protein; RAX1 protein, S cerevisiae; RSR1 protein, S cerevisiae; Saccharomyces cerevisiae protein; Article; binding competition; cell polarity; cell tracking; cytokinesis; live cell imaging; mating; microfluidics; microscopy; mitosis; nonhuman; polarization; priority journal; Saccharomyces cerevisiae; signal transduction; wild type; cell cycle; cell polarity; chemotaxis; cytology; genetics; metabolism; mutation; Saccharomyces cerevisiae; cdc42 GTP-Binding Protein, Saccharomyces cerevisiae; Cell Cycle; Cell Polarity; Chemotaxis; Cytokinesis; Mating Factor; Membrane Proteins; Mutation; rab GTP-Binding Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Signal Transduction
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160306
作者单位	Vasen, G., Department of Physiology, Molecular and Cellular Biology, School of Exact and Natural Sciences, University of Buenos Aires (UBA), Buenos Aires, C1428EGA, Argentina, Institute of Physiology, Molecular Biology and Neurosciences, National Council of Scientific and Technical Research, (IFIBYNE-UBACONICET), Buenos Aires, C1428EGA, Argentina; Dunayevich, P., Department of Physiology, Molecular and Cellular Biology, School of Exact and Natural Sciences, University of Buenos Aires (UBA), Buenos Aires, C1428EGA, Argentina, Institute of Physiology, Molecular Biology and Neurosciences, National Council of Scientific and Technical Research, (IFIBYNE-UBACONICET), Buenos Aires, C1428EGA, Argentina; Colman-Lerner, A., Department of Physiology, Molecular and Cellular Biology, School of Exact and Natural Sciences, University of Buenos Aires (UBA), Buenos Aires, C1428EGA, Argentina, Institute of Physiology, Molecular Biology and Neurosciences, National Council of Scientific and Technical Research, (IFIBYNE-UBACO...
推荐引用方式 GB/T 7714	Vasen G.,Dunayevich P.,Colman-Lerner A.. Mitotic and pheromone-specific intrinsic polarization cues interfere with gradient sensing in Saccharomyces cerevisiae[J],2020,117(12).
APA	Vasen G.,Dunayevich P.,&Colman-Lerner A..(2020).Mitotic and pheromone-specific intrinsic polarization cues interfere with gradient sensing in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America,117(12).
MLA	Vasen G.,et al."Mitotic and pheromone-specific intrinsic polarization cues interfere with gradient sensing in Saccharomyces cerevisiae".Proceedings of the National Academy of Sciences of the United States of America 117.12(2020).