气候变化领域集成服务门户(CCPortal): Distinct activities of Scrib module proteins organize epithelial polarity

CCPortal

DOI	10.1073/pnas.1918462117
	Distinct activities of Scrib module proteins organize epithelial polarity
	Khoury M.J.; Bilder D.
发表日期	2020
ISSN	0027-8424
卷号	117 期号:21
英文摘要	A polarized architecture is central to both epithelial structure and function. In many cells, polarity involves mutual antagonism between the Par complex and the Scribble (Scrib) module. While molecular mechanisms underlying Par-mediated apical determination are well-understood, how Scrib module proteins specify the basolateral domain remains unknown. Here, we demonstrate dependent and independent activities of Scrib, Discs-large (Dlg), and Lethal giant larvae (Lgl) using the Drosophila follicle epithelium. Our data support a linear hierarchy for localization, but rule out previously proposed protein-protein interactions as essential for polarization. Cortical recruitment of Scrib does not require palmitoylation or polar phospholipid binding but instead an independent cortically stabilizing activity of Dlg. Scrib and Dlg do not directly antagonize atypical protein kinase C (aPKC), but may instead restrict aPKC localization by enabling the aPKC-inhibiting activity of Lgl. Importantly, while Scrib, Dlg, and Lgl are each required, all three together are not sufficient to antagonize the Par complex. Our data demonstrate previously unappreciated diversity of function within the Scrib module and begin to define the elusive molecular functions of Scrib and Dlg. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Drosophila; Epithelia; Par complex; Polarity; Scrib module
语种	英语
scopus关键词	cell protein; phospholipid; protein kinase C; Scrib module protein; unclassified drug; aPKC protein, Drosophila; dlg1 protein, Drosophila; Drosophila protein; l(2)gl protein, Drosophila; membrane protein; protein kinase C; Scrib protein, Drosophila; tumor suppressor protein; Article; cell membrane; cell polarity; controlled study; Drosophila; epithelium cell; larva; nonhuman; palmitoylation; polarization; priority journal; protein localization; protein protein interaction; animal; cell polarity; cytology; Drosophila; epithelium; female; ovary follicle; physiology; Animals; Cell Polarity; Drosophila; Drosophila Proteins; Epithelial Cells; Epithelium; Female; Membrane Proteins; Ovarian Follicle; Protein Kinase C; Tumor Suppressor Proteins
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160288
作者单位	Khoury, M.J., Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, United States; Bilder, D., Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, United States
推荐引用方式 GB/T 7714	Khoury M.J.,Bilder D.. Distinct activities of Scrib module proteins organize epithelial polarity[J],2020,117(21).
APA	Khoury M.J.,&Bilder D..(2020).Distinct activities of Scrib module proteins organize epithelial polarity.Proceedings of the National Academy of Sciences of the United States of America,117(21).
MLA	Khoury M.J.,et al."Distinct activities of Scrib module proteins organize epithelial polarity".Proceedings of the National Academy of Sciences of the United States of America 117.21(2020).