气候变化领域集成服务门户(CCPortal): Wounding-induced changes in cellular pressure and localized auxin signalling spatially coordinate restorative divisions in roots

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DOI	10.1073/pnas.2003346117
	Wounding-induced changes in cellular pressure and localized auxin signalling spatially coordinate restorative divisions in roots
	Hoermayer L.; Montesinos J.C.; Marhava P.; Benková E.; Yoshida S.; Friml J.
发表日期	2020
ISSN	0027-8424
起始页码	15322
结束页码	15331
卷号	117 期号:26
英文摘要	Wound healing in plant tissues, consisting of rigid cell wallencapsulated cells, represents a considerable challenge and occurs through largely unknown mechanisms distinct from those in animals. Owing to their inability to migrate, plant cells rely on targeted cell division and expansion to regenerate wounds. Strict coordination of these wound-induced responses is essential to ensure efficient, spatially restricted wound healing. Single-cell tracking by live imaging allowed us to gain mechanistic insight into the wound perception and coordination of wound responses after laser-based wounding in Arabidopsis root. We revealed a crucial contribution of the collapse of damaged cells in wound perception and detected an auxin increase specific to cells immediately adjacent to the wound. This localized auxin increase balances woundinduced cell expansion and restorative division rates in a dosedependent manner, leading to tumorous overproliferation when the canonical TIR1 auxin signaling is disrupted. Auxin and woundinduced turgor pressure changes together also spatially define the activation of key components of regeneration, such as the transcription regulator ERF115. Our observations suggest that the wound signaling involves the sensing of collapse of damaged cells and a local auxin signaling activation to coordinate the downstream transcriptional responses in the immediate wound vicinity. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Auxin; Cell division; Cell expansion; ERF115; regeneration
语种	英语
scopus关键词	5-(4-chlorophenyl)-4H-1,2,4-triazole-3-thiol; alpha-naphthylphthalamic acid; Arabidopsis protein; indoleacetic acid derivative; kynurenine; phthalimide derivative; triazole derivative; Arabidopsis; cell division; drug effect; gene expression regulation; genetics; laser; metabolism; physiology; plant cell; plant root; regeneration; signal transduction; Arabidopsis; Arabidopsis Proteins; Cell Division; Gene Expression Regulation, Plant; Indoleacetic Acids; Kynurenine; Lasers; Phthalimides; Plant Cells; Plant Roots; Regeneration; Signal Transduction; Triazoles
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160276
作者单位	Hoermayer, L., Institute of Science and Technology Austria, Klosterneuburg, 3400, Austria; Montesinos, J.C., Department of Plant Molecular Biology, University of Lausanne, Lausanne, CH-1015, Switzerland; Marhava, P., Institute of Science and Technology Austria, Klosterneuburg, 3400, Austria; Benková, E., Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Cologne, 50829, Germany; Yoshida, S., Institute of Science and Technology Austria, Klosterneuburg, 3400, Austria; Friml, J., Institute of Science and Technology Austria, Klosterneuburg, 3400, Austria
推荐引用方式 GB/T 7714	Hoermayer L.,Montesinos J.C.,Marhava P.,et al. Wounding-induced changes in cellular pressure and localized auxin signalling spatially coordinate restorative divisions in roots[J],2020,117(26).
APA	Hoermayer L.,Montesinos J.C.,Marhava P.,Benková E.,Yoshida S.,&Friml J..(2020).Wounding-induced changes in cellular pressure and localized auxin signalling spatially coordinate restorative divisions in roots.Proceedings of the National Academy of Sciences of the United States of America,117(26).
MLA	Hoermayer L.,et al."Wounding-induced changes in cellular pressure and localized auxin signalling spatially coordinate restorative divisions in roots".Proceedings of the National Academy of Sciences of the United States of America 117.26(2020).