气候变化领域集成服务门户(CCPortal): Surface sensing stimulates cellular differentiation in Caulobacter crescentus

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DOI	10.1073/pnas.1920291117
	Surface sensing stimulates cellular differentiation in Caulobacter crescentus
	Snyder R.A.; Ellison C.K.; Severin G.B.; Whitfield G.B.; Waters C.M.; Brun Y.V.
发表日期	2020
ISSN	0027-8424
起始页码	17984
结束页码	17991
卷号	117 期号:30
英文摘要	Cellular differentiation is a fundamental strategy used by cells to generate specialized functions at specific stages of development. The bacterium Caulobacter crescentus employs a specialized dimorphic life cycle consisting of two differentiated cell types. How environmental cues, including mechanical inputs such as contact with a surface, regulate this cell cycle remain unclear. Here, we find that surface sensing by the physical perturbation of retracting extracellular pilus filaments accelerates cell-cycle progression and cellular differentiation. We show that physical obstruction of dynamic pilus activity by chemical perturbation or by a mutation in the outer-membrane pilus secretin CpaC stimulates early initiation of chromosome replication. In addition, we find that surface contact stimulates cell-cycle progression by demonstrating that surface-stimulated cells initiate early chromosome replication to the same extent as planktonic cells with obstructed pilus activity. Finally, we show that obstruction of pilus retraction stimulates the synthesis of the cell-cycle regulator cyclic diguanylate monophosphate (c-di-GMP) through changes in the activity and localization of two key regulatory histidine kinases that control cell fate and differentiation. Together, these results demonstrate that surface contact and sensing by alterations in pilus activity stimulate C. crescentus to bypass its developmentally programmed temporal delay in cell differentiation to more quickly adapt to a surface-associated lifestyle. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Bacteria; Cell cycle; Cellular differentiation; Surface sensing; Type IV pili
语种	英语
scopus关键词	protein histidine kinase; secretin; secretin CpaC; unclassified drug; bis(3',5')-cyclic diguanylic acid; cyclic GMP; Article; bacterium pilus; Caulobacter vibrioides; cell cycle progression; cell differentiation; cell fate; chromosome replication; controlled study; DNA replication; enzyme activity; enzyme localization; nonhuman; priority journal; bacterial phenomena and functions; biological model; Caulobacter vibrioides; cell cycle; fimbria; Gram negative infection; metabolism; microbiology; mutation; physiology; Bacterial Physiological Phenomena; Caulobacter crescentus; Cell Cycle; Cyclic GMP; DNA Replication; Fimbriae, Bacterial; Gram-Negative Bacterial Infections; Models, Biological; Mutation
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160249
作者单位	Snyder, R.A., Department of Biology, Indiana University, Bloomington, IN 47405, United States; Ellison, C.K., Department of Biology, Indiana University, Bloomington, IN 47405, United States; Severin, G.B., Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48823, United States; Whitfield, G.B., Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, QC H3C 3J7, Canada; Waters, C.M., Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48823, United States; Brun, Y.V., Department of Biology, Indiana University, Bloomington, IN 47405, United States, Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, QC H3C 3J7, Canada
推荐引用方式 GB/T 7714	Snyder R.A.,Ellison C.K.,Severin G.B.,et al. Surface sensing stimulates cellular differentiation in Caulobacter crescentus[J],2020,117(30).
APA	Snyder R.A.,Ellison C.K.,Severin G.B.,Whitfield G.B.,Waters C.M.,&Brun Y.V..(2020).Surface sensing stimulates cellular differentiation in Caulobacter crescentus.Proceedings of the National Academy of Sciences of the United States of America,117(30).
MLA	Snyder R.A.,et al."Surface sensing stimulates cellular differentiation in Caulobacter crescentus".Proceedings of the National Academy of Sciences of the United States of America 117.30(2020).