气候变化领域集成服务门户(CCPortal): Selective translation by alternative bacterial ribosomes

CCPortal

DOI	10.1073/pnas.2009607117
	Selective translation by alternative bacterial ribosomes
	Chen Y.-X.; Xu Z.-Y.; Ge X.; Sanyal S.; Lu Z.J.; Javid B.
发表日期	2020
ISSN	0027-8424
起始页码	19487
结束页码	19496
卷号	117 期号:32
英文摘要	Alternative ribosome subunit proteins are prevalent in the genomes of diverse bacterial species, but their functional significance is controversial. Attempts to study microbial ribosomal heterogeneity have mostly relied on comparing wild-type strains with mutants in which subunits have been deleted, but this approach does not allow direct comparison of alternate ribosome isoforms isolated from identical cellular contexts. Here, by simultaneously purifying canonical and alternative RpsR ribosomes from Mycobacterium smegmatis, we show that alternative ribosomes have distinct translational features compared with their canonical counterparts. Both alternative and canonical ribosomes actively take part in protein synthesis, although they translate a subset of genes with differential efficiency as measured by ribosome profiling. We also show that alternative ribosomes have a relative defect in initiation complex formation. Furthermore, a strain of M. smegmatis in which the alternative ribosome protein operon is deleted grows poorly in iron-depleted medium, uncovering a role for alternative ribosomes in iron homeostasis. Our work confirms the distinct and nonredundant contribution of alternative bacterial ribosomes for adaptation to hostile environments. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Alternative ribosomes; Mycobacterium; Ribosome profiling
语种	英语
scopus关键词	Article; bacterial strain; codon; complex formation; controlled study; iron homeostasis; Mycobacterium smegmatis; nonhuman; operon; phenotype; priority journal; protein synthesis; ribosome; RNA translation; wild type; genetics; growth, development and aging; metabolism; Mycobacterium smegmatis; ribosomal subunit; ribosome; translation initiation; bacterial protein; iron; ribosomal protein S18; ribosome protein; Bacterial Proteins; Iron; Mycobacterium smegmatis; Peptide Chain Initiation, Translational; Protein Biosynthesis; Ribosomal Proteins; Ribosome Subunits; Ribosomes
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160239
作者单位	Chen, Y.-X., Centre for Global Health and Infectious Diseases, Collaborative Innovation Centre for the Diagnosis and Treatment of Infectious Diseases, Tsinghua University, School of Medicine, Beijing, 100084, China, Division of Experimental Medicine, University of California, San Francisco, CA 94110, United States; Xu, Z.-Y., Centre for Global Health and Infectious Diseases, Collaborative Innovation Centre for the Diagnosis and Treatment of Infectious Diseases, Tsinghua University, School of Medicine, Beijing, 100084, China, Ministry of Education Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China; Ge, X., Department of Cell and Molecular Biology, Uppsala University, Uppsala, 75124, Sweden; Sanyal, S., Department of Cell and Molecular Biology, Uppsala University, Uppsala, 75124, Sweden; Lu, Z.J., Ministry of Education Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Scien...
推荐引用方式 GB/T 7714	Chen Y.-X.,Xu Z.-Y.,Ge X.,et al. Selective translation by alternative bacterial ribosomes[J],2020,117(32).
APA	Chen Y.-X.,Xu Z.-Y.,Ge X.,Sanyal S.,Lu Z.J.,&Javid B..(2020).Selective translation by alternative bacterial ribosomes.Proceedings of the National Academy of Sciences of the United States of America,117(32).
MLA	Chen Y.-X.,et al."Selective translation by alternative bacterial ribosomes".Proceedings of the National Academy of Sciences of the United States of America 117.32(2020).