气候变化领域集成服务门户(CCPortal): RNA abasic sites in yeast and human cells

CCPortal

DOI	10.1073/pnas.2011511117
	RNA abasic sites in yeast and human cells
	Liu Y.; Rodriguez Y.; Ross R.L.; Zhao R.; Watts J.A.; Grunseich C.; Bruzel A.; Li D.; Burdick J.T.; Prasad R.; Crouch R.J.; Limbach P.A.; Wilson S.H.; Cheung V.G.
发表日期	2020
ISSN	0027-8424
起始页码	20689
结束页码	20695
卷号	117 期号:34
英文摘要	RNA abasic sites and the mechanisms involved in their regulation are mostly unknown; in contrast, DNA abasic sites are well-studied. We found surprisingly that, in yeast and human cells, RNA abasic sites are prevalent. When a base is lost from RNA, the remaining ribose is found as a closed-ring or an open-ring sugar with a reactive C1′ aldehyde group. Using primary amine-based reagents that react with the aldehyde group, we uncovered evidence for abasic sites in nascent RNA, messenger RNA, and ribosomal RNA from yeast and human cells. Mass spectroscopic analysis confirmed the presence of RNA abasic sites. The RNA abasic sites were found to be coupled to R-loops. We show that human methylpurine DNA glycosylase cleaves N-glycosidic bonds on RNA and that human apurinic/apyrimidinic endonuclease 1 incises RNA abasic sites in RNA–DNA hybrids. Our results reveal that, in yeast and human cells, there are RNA abasic sites, and we identify a glycosylase that generates these sites and an AP endonuclease that processes them. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Apurinic/apyrimidinic endonuclease 1; Methylpurine DNA glycosylase; R-loop; RNA; RNA abasic site
语种	英语
scopus关键词	deoxyribonuclease I; DNA glycosyltransferase; messenger RNA; ribosome RNA; deoxyribonuclease I; DNA; DNA (apurinic or apyrimidinic site) lyase; DNA glycosyltransferase; nucleotide; RNA; Article; base excision repair; biotinylation; chemical bond; controlled study; DNA RNA hybridization; electrophilicity; fungal strain; human; human cell; mass spectrometry; nonhuman; priority journal; protein RNA binding; RNA processing; Saccharomyces cerevisiae; scanning electron microscopy; yeast cell; binding site; chemistry; DNA damage; DNA repair; enzyme specificity; genetics; metabolism; nucleotide sequence; Saccharomyces cerevisiae; yeast; Base Sequence; Binding Sites; Deoxyribonuclease I; DNA; DNA Damage; DNA Glycosylases; DNA Repair; DNA-(Apurinic or Apyrimidinic Site) Lyase; Humans; Nucleotides; R-Loop Structures; RNA; Saccharomyces cerevisiae; Substrate Specificity; Yeasts
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160236
作者单位	Liu, Y., HHMI, University of Michigan, Ann Arbor, MI 48109, United States, Department of Pediatrics, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, United States; Rodriguez, Y., National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, United States; Ross, R.L., Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, United States; Zhao, R., Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, United States; Watts, J.A., Department of Medicine, University of Michigan, Ann Arbor, MI 48109, United States; Grunseich, C., National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD 20892, United States; Bruzel, A., HHMI, University of Michigan, Ann Arbor, MI 48109, United States, Department of Pediatrics, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, United States; Li, D., HHMI, U...
推荐引用方式 GB/T 7714	Liu Y.,Rodriguez Y.,Ross R.L.,et al. RNA abasic sites in yeast and human cells[J],2020,117(34).
APA	Liu Y..,Rodriguez Y..,Ross R.L..,Zhao R..,Watts J.A..,...&Cheung V.G..(2020).RNA abasic sites in yeast and human cells.Proceedings of the National Academy of Sciences of the United States of America,117(34).
MLA	Liu Y.,et al."RNA abasic sites in yeast and human cells".Proceedings of the National Academy of Sciences of the United States of America 117.34(2020).