气候变化领域集成服务门户(CCPortal): Maize ANT1 modulates vascular development; chloroplast development; photosynthesis; and plant growth

CCPortal

DOI	10.1073/pnas.2004091117
	Maize ANT1 modulates vascular development; chloroplast development; photosynthesis; and plant growth
	Liu W.-Y.; Lin H.-H.; Yu C.-P.; Chang C.-K.; Chen H.-J.; Lin J.-J.; Lu M.-Y.J.; Tu S.-L.; Shiu S.-H.; Wu S.-H.; Ku M.S.B.; Li W.-H.
发表日期	2020
ISSN	0027-8424
起始页码	21747
结束页码	21756
卷号	117 期号:35
英文摘要	Arabidopsis AINTEGUMENTA (ANT), an AP2 transcription factor, is known to control plant growth and floral organogenesis. In this study, our transcriptome analysis and in situ hybridization assays of maize embryonic leaves suggested that maize ANT1 (ZmANT1) regulates vascular development. To better understand ANT1 functions, we determined the binding motif of ZmANT1 and then showed that ZmANT1 binds the promoters of millet SCR1, GNC, and AN3, which are key regulators of Kranz anatomy, chloroplast development, and plant growth, respectively. We generated a mutant with a single-codon deletion and two frameshift mutants of the ANT1 ortholog in the C4millet Setaria viridis by the CRISPR/Cas9 technique. The two frameshift mutants displayed reduced photosynthesis efficiency and growth rate, smaller leaves, and lower grain yields than wild-type (WT) plants. Moreover, their leaves sporadically exhibited distorted Kranz anatomy and vein spacing. Conducting transcriptomic analysis of developing leaves in the WT and the three mutants we identified differentially expressed genes (DEGs) in the two frameshift mutant lines and found many downregulated DEGs enriched in photosynthesis, heme, tetrapyrrole binding, and antioxidant activity. In addition, we predicted many target genes of ZmANT1 and chose 13 of them to confirm binding of ZmANT1 to their promoters. Based on the above observations, we proposed a model for ANT1 regulation of cell proliferation and leaf growth, vascular and vein development, chloroplast development, and photosynthesis through its target genes. Our study revealed biological roles of ANT1 in several developmental processes beyond its known roles in plant growth and floral organogenesis. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	AINTEGUMENTA; Chloroplast development; Kranz anatomy; Photosynthesis; Vascular development
语种	英语
scopus关键词	ANT1 protein; heme; tetrapyrrole derivative; transcription factor; unclassified drug; adenine nucleotide translocase; amino acid transporter; plant protein; transcription factor; transcriptome; AN3 gene; angiogenesis; ANT1 gene; antioxidant activity; Article; cell proliferation; chloroplast; controlled study; CRISPR-CAS9 system; down regulation; frameshift mutation; gene; gene expression; GNC gene; grain yield; leaf morphology; maize; millet; mutant; nonhuman; photosynthesis; plant growth; priority journal; promoter region; protein binding; protein motif; SCR1 gene; wild type; chloroplast; flower; gene expression profiling; gene expression regulation; genetics; growth, development and aging; maize; metabolism; photosynthesis; physiology; plant development; plant leaf; plant organogenesis; ANT1 gene; cell maturation; codon; gene control; gene deletion; gene function; gene targeting; genetic analysis; plant gene; prediction; Setaria viridis; transcriptomics; vein formation (physiology); Adenine Nucleotide Translocator 1; Amino Acid Transport Systems, Neutral; Chloroplasts; Flowers; Gene Expression Profiling; Gene Expression Regulation, Plant; Millets; Organogenesis, Plant; Photosynthesis; Plant Development; Plant Leaves; Plant Proteins; Transcription Factors; Transcriptome; Zea mays
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160233
作者单位	Liu, W.-Y., Biodiversity Research Center, Academia Sinica, Taipei, 115, Taiwan; Lin, H.-H., Biodiversity Research Center, Academia Sinica, Taipei, 115, Taiwan, Department of Horticulture and Biotechnology, Chinese Culture University, Taipei, 111, Taiwan; Yu, C.-P., Biodiversity Research Center, Academia Sinica, Taipei, 115, Taiwan; Chang, C.-K., Biodiversity Research Center, Academia Sinica, Taipei, 115, Taiwan; Chen, H.-J., Biodiversity Research Center, Academia Sinica, Taipei, 115, Taiwan; Lin, J.-J., Biodiversity Research Center, Academia Sinica, Taipei, 115, Taiwan; Lu, M.-Y.J., Biodiversity Research Center, Academia Sinica, Taipei, 115, Taiwan; Tu, S.-L., Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 115, Taiwan; Shiu, S.-H., Department of Plant Biology, Michigan State University, East Lansing, MI 48824, United States, Department of Computational Mathematics, Science, and Engineering, Michigan State University, East Lansing, MI 48824, United States; Wu, S.-H., Institute of Plan...
推荐引用方式 GB/T 7714	Liu W.-Y.,Lin H.-H.,Yu C.-P.,et al. Maize ANT1 modulates vascular development; chloroplast development; photosynthesis; and plant growth[J],2020,117(35).
APA	Liu W.-Y..,Lin H.-H..,Yu C.-P..,Chang C.-K..,Chen H.-J..,...&Li W.-H..(2020).Maize ANT1 modulates vascular development; chloroplast development; photosynthesis; and plant growth.Proceedings of the National Academy of Sciences of the United States of America,117(35).
MLA	Liu W.-Y.,et al."Maize ANT1 modulates vascular development; chloroplast development; photosynthesis; and plant growth".Proceedings of the National Academy of Sciences of the United States of America 117.35(2020).