气候变化领域集成服务门户(CCPortal): Root angle modifications by the DRO1 homolog improve rice yields in saline paddy fields

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DOI	10.1073/pnas.2005911117
	Root angle modifications by the DRO1 homolog improve rice yields in saline paddy fields
	Kitomi Y.; Hanzawa E.; Kuya N.; Inoue H.; Hara N.; Kawai S.; Kanno N.; Endo M.; Sugimoto K.; Yamazaki T.; Sakamoto S.; Sentoku N.; Wu J.; Kanno H.; Mitsuda N.; Toriyama K.; Sato T.; Uga Y.
发表日期	2020
ISSN	0027-8424
起始页码	21242
结束页码	21250
卷号	117 期号:35
英文摘要	The root system architecture (RSA) of crops can affect their production, particularly in abiotic stress conditions, such as with drought, waterlogging, and salinity. Salinity is a growing problem worldwide that negatively impacts on crop productivity, and it is believed that yields could be improved if RSAs that enabled plants to avoid saline conditions were identified. Here, we have demonstrated, through the cloning and characterization of qSOR1 (quantitative trait locus for SOIL SURFACE ROOTING 1), that a shallower root growth angle (RGA) could enhance rice yields in saline paddies. qSOR1 is negatively regulated by auxin, predominantly expressed in root columella cells, and involved in the gravitropic responses of roots. qSOR1 was found to be a homolog of DRO1 (DEEPER ROOTING 1), which is known to control RGA. CRISPR-Cas9 assays revealed that other DRO1 homologs were also involved in RGA. Introgression lines with combinations of gain-of-function and loss-of-function alleles in qSOR1 and DRO1 demonstrated four different RSAs (ultra-shallow, shallow, intermediate, and deep rooting), suggesting that natural alleles of the DRO1 homologs could be utilized to control RSA variations in rice. In saline paddies, near-isogenic lines carrying the qSOR1 loss-of-function allele had soil-surface roots (SOR) that enabled rice to avoid the reducing stresses of saline soils, resulting in increased yields compared to the parental cultivars without SOR. Our findings suggest that DRO1 homologs are valuable targets for RSA breeding and could lead to improved rice production in environments characterized by abiotic stress. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Abiotic stress; Gravitropism; Oryza sativa L; Quantitative trait locus (QTL); Root trait
语种	英语
scopus关键词	abiotic stress; Article; carboxy terminal sequence; controlled study; CRISPR-CAS9 system; DRO1 gene; gene expression; gene frequency; genetic variability; genotype; gravitropism; limit of quantitation; loss of function mutation; molecular cloning; nonhuman; paddy soil; phenotype; plant gene; plant root; priority journal; qSOR1; rice; root growth angle; signal transduction; soil; soil surface root; allele; drought; genetics; growth, development and aging; metabolism; Oryza; quantitative trait locus; Arabidopsis protein; AT1G72490 protein, Arabidopsis; indoleacetic acid derivative; nuclear protein; Alleles; Arabidopsis Proteins; Droughts; Indoleacetic Acids; Nuclear Proteins; Oryza; Phenotype; Plant Roots; Quantitative Trait Loci
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160231
作者单位	Kitomi, Y., Institute of Crop Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-8518, Japan; Hanzawa, E., Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980-8577, Japan; Kuya, N., Institute of Crop Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-8518, Japan; Inoue, H., Institute of Agrobiological Sciences, NARO, Tsukuba, Ibaraki 305-8634, Japan, PRESTO, Japan Science and Technology Agency (JST), Kawaguchi, Saitama 332-0012, Japan; Hara, N., Institute of Agrobiological Sciences, NARO, Tsukuba, Ibaraki 305-8634, Japan; Kawai, S., Institute of Crop Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-8518, Japan, WDB Co., Ltd., Tsukuba, 305-0032, Japan; Kanno, N., Institute of Crop Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-8518, Japan; Endo, M., Institute of Agrobiological Sciences, NARO, Tsukuba, Ibaraki 305-8634,...
推荐引用方式 GB/T 7714	Kitomi Y.,Hanzawa E.,Kuya N.,et al. Root angle modifications by the DRO1 homolog improve rice yields in saline paddy fields[J],2020,117(35).
APA	Kitomi Y..,Hanzawa E..,Kuya N..,Inoue H..,Hara N..,...&Uga Y..(2020).Root angle modifications by the DRO1 homolog improve rice yields in saline paddy fields.Proceedings of the National Academy of Sciences of the United States of America,117(35).
MLA	Kitomi Y.,et al."Root angle modifications by the DRO1 homolog improve rice yields in saline paddy fields".Proceedings of the National Academy of Sciences of the United States of America 117.35(2020).