气候变化领域集成服务门户(CCPortal): EPSIN1 and MTV1 define functionally overlapping but molecularly distinct trans-Golgi network subdomains in Arabidopsis

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DOI	10.1073/pnas.2004822117
	EPSIN1 and MTV1 define functionally overlapping but molecularly distinct trans-Golgi network subdomains in Arabidopsis
	Heinze L.; Freimuth N.; Rößling A.-K.; Hahnke R.; Riebschläger S.; Fröhlich A.; Sampathkumar A.; McFarlane H.E.; Sauer M.
发表日期	2020
ISSN	0027-8424
起始页码	25880
结束页码	25889
卷号	117 期号:41
英文摘要	The plant trans-Golgi network (TGN) is a central trafficking hub where secretory, vacuolar, recycling, and endocytic pathways merge. Among currently known molecular players involved in TGN transport, three different adaptor protein (AP) complexes promote vesicle generation at the TGN with different cargo specificity and destination. Yet, it remains unresolved how sorting into diverging vesicular routes is spatially organized. Here, we study the family of Arabidopsis thaliana Epsin-like proteins, which are accessory proteins to APs facilitating vesicle biogenesis. By comprehensive molecular, cellular, and genetic analysis of the EPSIN gene family, we identify EPSIN1 and MODIFIED TRANSPORT TO THE VACUOLE1 (MTV1) as its only TGN-associated members. Despite their large phylogenetic distance, they perform overlapping functions in vacuolar and secretory transport. By probing their relationship with AP complexes, we find that they define two molecularly independent pathways:While EPSIN1 associates with AP-1, MTV1 interacts with AP-4, whose function is required for MTV1 recruitment. Although both EPSIN1/AP-1 and MTV1/AP-4 pairs reside at the TGN, high-resolution microscopy reveals them as spatially separate entities. Our results strongly support the hypothesis of molecularly, functionally, and spatially distinct subdomains of the plant TGN and suggest that functional redundancy can be achieved through parallelization of molecularly distinct but functionally overlapping pathways. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Adaptor complexes; ENTH domain; Epsins; Intracellular protein transport; Trans-Golgi network
语种	英语
scopus关键词	adaptor protein; epsin 1; membrane protein; modified transport to the vacuole 1; unclassified drug; Arabidopsis; Article; biogenesis; controlled study; genetic analysis; nonhuman; phylogeny; priority journal; protein protein interaction; protein transport; trans Golgi network
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160211
作者单位	Heinze, L., Department of Plant Physiology, University of Potsdam, Potsdam, 14476, Germany; Freimuth, N., Department of Plant Physiology, University of Potsdam, Potsdam, 14476, Germany; Rößling, A.-K., Department of Plant Physiology, University of Potsdam, Potsdam, 14476, Germany; Hahnke, R., Department of Plant Physiology, University of Potsdam, Potsdam, 14476, Germany; Riebschläger, S., Department of Plant Physiology, University of Potsdam, Potsdam, 14476, Germany; Fröhlich, A., Max Planck Institute of Molecular Plant Physiology, Potsdam, 14476, Germany; Sampathkumar, A., Max Planck Institute of Molecular Plant Physiology, Potsdam, 14476, Germany; McFarlane, H.E., School of Biosciences, University of Melbourne, Melbourne, VIC 3010, Australia, Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3G5, Canada; Sauer, M., Department of Plant Physiology, University of Potsdam, Potsdam, 14476, Germany
推荐引用方式 GB/T 7714	Heinze L.,Freimuth N.,Rößling A.-K.,et al. EPSIN1 and MTV1 define functionally overlapping but molecularly distinct trans-Golgi network subdomains in Arabidopsis[J],2020,117(41).
APA	Heinze L..,Freimuth N..,Rößling A.-K..,Hahnke R..,Riebschläger S..,...&Sauer M..(2020).EPSIN1 and MTV1 define functionally overlapping but molecularly distinct trans-Golgi network subdomains in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America,117(41).
MLA	Heinze L.,et al."EPSIN1 and MTV1 define functionally overlapping but molecularly distinct trans-Golgi network subdomains in Arabidopsis".Proceedings of the National Academy of Sciences of the United States of America 117.41(2020).