气候变化领域集成服务门户(CCPortal): A bacterial cytolinker couples positioning of magnetic organelles to cell shape control

CCPortal

DOI	10.1073/pnas.2014659117
	A bacterial cytolinker couples positioning of magnetic organelles to cell shape control
	Pfeiffer D.; Toro-Nahuelpan M.; Awal R.P.; Müller F.-D.; Bramkamp M.; Plitzko J.M.; Schüler D.
发表日期	2020
ISSN	0027-8424
起始页码	32086
结束页码	32097
卷号	117 期号:50
英文摘要	Magnetotactic bacteria maneuver within the geomagnetic field by means of intracellular magnetic organelles, magnetosomes, which are aligned into a chain and positioned at midcell by a dedicated magnetosome-specific cytoskeleton, the “magnetoskeleton.” However, how magnetosome chain organization and resulting magnetotaxis is linked to cell shape has remained elusive. Here, we describe the cytoskeletal determinant CcfM (curvature-inducing coiled-coil filament interacting with the magnetoskeleton), which links the magnetoskeleton to cell morphology regulation in Magnetospirillum gryphiswaldense. Membrane-anchored CcfM localizes in a filamentous pattern along regions of inner positive-cell curvature by its coiled-coil motifs, and independent of the magnetoskeleton. CcfM overexpression causes additional circumferential localization patterns, associated with a dramatic increase in cell curvature, and magnetosome chain mislocalization or complete chain disruption. In contrast, deletion of ccfM results in decreased cell curvature, impaired cell division, and predominant formation of shorter, doubled chains of magnetosomes. Pleiotropic effects of CcfM on magnetosome chain organization and cell morphology are supported by the finding that CcfM interacts with the magnetoskeleton-related MamY and the actin-like MamK via distinct motifs, and with the cell shape-related cytoskeleton via MreB. We further demonstrate that CcfM promotes motility and magnetic alignment in structured environments, and thus likely confers a selective advantage in natural habitats of magnetotactic bacteria, such as aquatic sediments. Overall, we unravel the function of a prokaryotic cytoskeletal constituent that is widespread in magnetic and nonmagnetic spirilla-shaped Alphaproteobacteria. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Cytoskeleton; Magnetosome; Magnetotaxis; MamK; MreB
语种	英语
scopus关键词	actin; binding protein; curvature inducing coiled coil filament interacting with the magnetoskeleton; cytoskeleton protein; MamK protein; MamY protein; MreB protein; unclassified drug; Article; ccfM gene; cell division; cell motility; cell shape; cell structure; cellular distribution; controlled study; gene; gene deletion; gene overexpression; magnetism; magnetosome; Magnetospirillum gryphiswaldense; nonhuman; pleiotropy; priority journal; protein motif
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160189
作者单位	Pfeiffer, D., Department of Microbiology, University Bayreuth, Bayreuth, 95447, Germany; Toro-Nahuelpan, M., Department of Microbiology, University Bayreuth, Bayreuth, 95447, Germany, Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Planegg-Martinsried, 82152, Germany; Awal, R.P., Department of Microbiology, University Bayreuth, Bayreuth, 95447, Germany; Müller, F.-D., Department of Microbiology, University Bayreuth, Bayreuth, 95447, Germany; Bramkamp, M., Institute for General Microbiology, Christian-Albrechts-University, Kiel, 24118, Germany; Plitzko, J.M., Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Planegg-Martinsried, 82152, Germany; Schüler, D., Department of Microbiology, University Bayreuth, Bayreuth, 95447, Germany
推荐引用方式 GB/T 7714	Pfeiffer D.,Toro-Nahuelpan M.,Awal R.P.,et al. A bacterial cytolinker couples positioning of magnetic organelles to cell shape control[J],2020,117(50).
APA	Pfeiffer D..,Toro-Nahuelpan M..,Awal R.P..,Müller F.-D..,Bramkamp M..,...&Schüler D..(2020).A bacterial cytolinker couples positioning of magnetic organelles to cell shape control.Proceedings of the National Academy of Sciences of the United States of America,117(50).
MLA	Pfeiffer D.,et al."A bacterial cytolinker couples positioning of magnetic organelles to cell shape control".Proceedings of the National Academy of Sciences of the United States of America 117.50(2020).