气候变化领域集成服务门户(CCPortal): Ötzi's last meals: DNA analysis of the intestinal content of the neolithic glacier mummy from the Alps

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DOI	10.1073/pnas.192184599
	Ötzi's last meals: DNA analysis of the intestinal content of the neolithic glacier mummy from the Alps
	Rollo F.; Ubaldi M.; Ermini L.; Marota I.
发表日期	2002
ISSN	0027-8424
起始页码	12594
结束页码	12599
卷号	99 期号:20
英文摘要	Samples of the intestinal content were collected from the ileum and colon of the Neolithic glacier mummy popularly known as the Tyrolean Iceman, or Ötzi. DNA was extracted from the samples and PCR amplified, using a variety of primer pairs designed to bind to different genes (mammal mitochondrial 12S ribosomal RNA gene, plant/fungal nuclear 18S ribosomal RNA gene, plant chloroplast ribulose bisphosphate carboxylase large subunit gene). This made it possible to distinguish between animal and plant food residues (macroremains) and pollen (microremains). According to the DNA reconstruction, the man's last meal was composed of red deer (Cervus elaphus) meat, and, possibly, cereals; this meal had been preceded by another one based on ibex (Capra ibex), different species of dicots, and cereals. The DNA spectrum corresponding to pollen residues in the colon, on the other hand, fits with the hypothesis that the last journey of the Neolithic hunter/warrior was made through a subalpine coniferous forest to the site at over 3,200 m above sea level, where his mummified body was to be discovered 5,000 years later.
英文关键词	Capra ibex; Cervus elaphus; Iceman
语种	英语
scopus关键词	ribulosebisphosphate carboxylase; RNA 18S; aspartic acid; DNA; ribosome RNA; vegetable protein; article; cereal; chloroplast; colon; controlled study; DNA determination; DNA extraction; DNA sequence; gene amplification; glacier; human; ileum; meal; meat; nucleotide sequence; paleopathology; pollen; polymerase chain reaction; priority journal; sequence analysis; animal; colon; fungal gene; gene; genetics; hominid; ileum; intestine; male; metabolism; molecular genetics; muscle; pathology; phylogeny; physiology; stomach juice; Animalia; Capra ibex; Cervidae; Cervus; Cervus elaphus; Dicotyledoneae; Mammalia; Animals; Aspartic Acid; Chloroplasts; Colon; DNA; Gastrointestinal Contents; Genes, Fungal; Genes, Plant; Hominidae; Humans; Ileum; Intestines; Male; Meat; Molecular Sequence Data; Mummies; Muscles; Phylogeny; Plant Proteins; Pollen; RNA, Ribosomal; RNA, Ribosomal, 18S
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/159201
作者单位	Rollo, F., Lab. di Archeo-Antropol. Molecolare, Università di Camerino, I-62032 Camerino, Italy; Ubaldi, M., Lab. di Archeo-Antropol. Molecolare, Università di Camerino, I-62032 Camerino, Italy; Ermini, L., Lab. di Archeo-Antropol. Molecolare, Università di Camerino, I-62032 Camerino, Italy; Marota, I., Lab. di Archeo-Antropol. Molecolare, Università di Camerino, I-62032 Camerino, Italy
推荐引用方式 GB/T 7714	Rollo F.,Ubaldi M.,Ermini L.,et al. Ötzi's last meals: DNA analysis of the intestinal content of the neolithic glacier mummy from the Alps[J],2002,99(20).
APA	Rollo F.,Ubaldi M.,Ermini L.,&Marota I..(2002).Ötzi's last meals: DNA analysis of the intestinal content of the neolithic glacier mummy from the Alps.Proceedings of the National Academy of Sciences of the United States of America,99(20).
MLA	Rollo F.,et al."Ötzi's last meals: DNA analysis of the intestinal content of the neolithic glacier mummy from the Alps".Proceedings of the National Academy of Sciences of the United States of America 99.20(2002).