气候变化领域集成服务门户(CCPortal): Resolving postglacial phylogeography using high-throughput sequencing

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DOI	10.1073/pnas.1006538107
	Resolving postglacial phylogeography using high-throughput sequencing
	Emerson K.J.; Merz C.R.; Catchen J.M.; Hohenlohe P.A.; Cresko W.A.; Bradshaw W.E.; Holzapfel C.M.
发表日期	2010
ISSN	0027-8424
起始页码	16196
结束页码	16200
卷号	107 期号:37
英文摘要	The distinction between model and nonmodel organisms is becoming increasingly blurred. High-throughput, second-generation sequencing approaches are being applied to organisms based on their interesting ecological, physiological, developmental, or evolutionary properties and not on the depth of genetic information available for them. Here, we illustrate this point using a low-cost, efficient technique to determine the fine-scale phylogenetic relationships among recently diverged populations in a species. This application of restriction site-associated DNA tags (RAD tags) reveals previously unresolved genetic structure and direction of evolution in the pitcher plant mosquito, Wyeomyia smithii, from a southern Appalachian Mountain refugium following recession of the Laurentide Ice Sheet at 22,000-19,000 B.P. The RAD tag method can be used to identify detailed patterns of phylogeography in any organism regardless of existing genomic data, and, more broadly, to identify incipient speciation and genome-wide variationin natural populations in general.
英文关键词	Genomics; Restriction site-associated DNA tag; Second-generation sequencing; Wyeomyia smithii
语种	英语
scopus关键词	article; gene sequence; gene structure; genetic analysis; genome; genotype; glacier; molecular evolution; mosquito; nucleotide sequence; phylogeny; phylogeography; priority journal; restriction site; species; technique; animal; chemistry; DNA sequence; genetics; molecular genetics; phylogeny; population genetics; procedures; sequence alignment; United States; Wyeomyia smithii; Animals; Appalachian Region; Base Sequence; Culicidae; Genetics, Population; Mid-Atlantic Region; Molecular Sequence Data; New England; Phylogeny; Sequence Alignment; Sequence Analysis, DNA
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/159152
作者单位	Emerson, K.J., Center for Ecology and Evolutionary Biology, University of Oregon, Eugene, OR 97403-5289, United States; Merz, C.R., Center for Ecology and Evolutionary Biology, University of Oregon, Eugene, OR 97403-5289, United States; Catchen, J.M., Center for Ecology and Evolutionary Biology, University of Oregon, Eugene, OR 97403-5289, United States; Hohenlohe, P.A., Center for Ecology and Evolutionary Biology, University of Oregon, Eugene, OR 97403-5289, United States; Cresko, W.A., Center for Ecology and Evolutionary Biology, University of Oregon, Eugene, OR 97403-5289, United States; Bradshaw, W.E., Center for Ecology and Evolutionary Biology, University of Oregon, Eugene, OR 97403-5289, United States; Holzapfel, C.M., Center for Ecology and Evolutionary Biology, University of Oregon, Eugene, OR 97403-5289, United States
推荐引用方式 GB/T 7714	Emerson K.J.,Merz C.R.,Catchen J.M.,et al. Resolving postglacial phylogeography using high-throughput sequencing[J],2010,107(37).
APA	Emerson K.J..,Merz C.R..,Catchen J.M..,Hohenlohe P.A..,Cresko W.A..,...&Holzapfel C.M..(2010).Resolving postglacial phylogeography using high-throughput sequencing.Proceedings of the National Academy of Sciences of the United States of America,107(37).
MLA	Emerson K.J.,et al."Resolving postglacial phylogeography using high-throughput sequencing".Proceedings of the National Academy of Sciences of the United States of America 107.37(2010).