气候变化领域集成服务门户(CCPortal): Hyperprolinemic larvae of the drosophilid fly; Chymomyza costata; survive cryopreservation in liquid nitrogen

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DOI	10.1073/pnas.1107060108
	Hyperprolinemic larvae of the drosophilid fly; Chymomyza costata; survive cryopreservation in liquid nitrogen
	Koštál V.; Zahradníèková H.; Šimek P.
发表日期	2011
ISSN	0027-8424
起始页码	13041
结束页码	13046
卷号	108 期号:32
英文摘要	The larva of the drosophilid fly, Chymomyza costata, is probably the most complex metazoan organism that can survive submergence in liquid nitrogen (-196 °C) in a fully hydrated state. We examined the associations between the physiological and biochemical parameters of differently acclimated larvae and their freeze tolerance. Entering diapause is an essential and sufficient prerequisite for attaining high levels of survival in liquid nitrogen (23% survival to adult stage), although cold acclimation further improves this capacity (62% survival). Profiling of 61 different metabolites identified proline as a prominent compound whose concentration increased from 20 to 147 mM during diapause transition and subsequent cold acclimation. This study provides direct evidence for the essential role of proline in high freeze tolerance. We increased the levels of proline in the larval tissues by feeding larvae proline-augmented diets and found that this simple treatment dramatically improved their freeze tolerance. Cell and tissue survival following exposure to liquid nitrogen was evident in proline-fed nondiapause larvae, and survival to adult stage increased from 0% to 36% in proline-fed diapause-destined larvae. A significant statistical correlation was found between the whole-body concentration of proline, either natural or artificial, and survival to the adult stage in liquid nitrogen for diapause larvae. Differential scanning calorimetry analysis suggested that high proline levels, in combination with a relatively low content of osmotically active water and freeze dehydration, increased the propensity of the remaining unfrozen water to undergo a glass-like transition (vitrification) and thus facilitated the prevention of cryoinjury.
英文关键词	Cold hardiness; Glass transition; Insect freeze tolerance; Metabolomics
语种	英语
scopus关键词	liquid nitrogen; proline; article; biochemistry; cell survival; cold acclimatization; controlled study; cryopreservation; dehydration; diapause; diet; differential scanning calorimetry; Drosophilidae; examination; larva; metabolite; osmosis; physiology; priority journal; survival; tissue survival; vitrification; Acclimatization; Adaptation, Physiological; Amino Acid Metabolism, Inborn Errors; Animals; Body Water; Calorimetry, Differential Scanning; Cryopreservation; Diet; Drosophilidae; Feeding Behavior; Freezing; Glass; Larva; Nitrogen; Osmosis; Principal Component Analysis; Proline; Proline Oxidase; Survival Analysis; Chymomyza costata; Drosophilidae; Hexapoda; Metazoa
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/159001
作者单位	Koštál, V., Institute of Entomology, Biology Centre, Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 České Budějovice, Czech Republic, Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic; Zahradníèková, H., Institute of Entomology, Biology Centre, Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 České Budějovice, Czech Republic; Šimek, P., Institute of Entomology, Biology Centre, Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 České Budějovice, Czech Republic
推荐引用方式 GB/T 7714	Koštál V.,Zahradníèková H.,Šimek P.. Hyperprolinemic larvae of the drosophilid fly; Chymomyza costata; survive cryopreservation in liquid nitrogen[J],2011,108(32).
APA	Koštál V.,Zahradníèková H.,&Šimek P..(2011).Hyperprolinemic larvae of the drosophilid fly; Chymomyza costata; survive cryopreservation in liquid nitrogen.Proceedings of the National Academy of Sciences of the United States of America,108(32).
MLA	Koštál V.,et al."Hyperprolinemic larvae of the drosophilid fly; Chymomyza costata; survive cryopreservation in liquid nitrogen".Proceedings of the National Academy of Sciences of the United States of America 108.32(2011).