气候变化领域集成服务门户(CCPortal): Gene expression changes governing extreme dehydration tolerance in an Antarctic insect

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DOI	10.1073/pnas.1218661109
	Gene expression changes governing extreme dehydration tolerance in an Antarctic insect
	Teets N.M.; Peyton J.T.; Colinet H.; Renault D.; Kelley J.L.; Kawarasaki Y.; Lee R.E.; Jr.; Denlinger D.L.
发表日期	2012
ISSN	0027-8424
起始页码	20744
结束页码	20749
卷号	109 期号:50
英文摘要	Among terrestrial organisms, arthropods are especially susceptible to dehydration, given their small body size and high surface area to volume ratio. This challenge is particularly acute for polar arthropods that face near-constant desiccating conditions, as water is frozen and thus unavailable for much of the year. The molecular mechanisms that govern extreme dehydration tolerance in insects remain largely undefined. In this study, we used RNA sequencing to quantify transcriptional mechanisms of extreme dehydration tolerance in the Antarctic midge, Belgica antarctica, the world’s southernmost insect and only insect endemic to Antarctica. Larvae of B. antarctica are remarkably tolerant of dehydration, surviving losses up to 70% of their body water. Gene expression changes in response to dehydration indicated up-regulation of cellular recycling pathways including the ubiquitin-mediated proteasome and autophagy, with concurrent down-regulation of genes involved in general metabolism and ATP production. Metabolomics results revealed shifts in metabolite pools that correlated closely with changes in gene expression, indicating that coordinated changes in gene expression and metabolism are a critical component of the dehydration response. Finally, using comparative genomics, we compared our gene expression results with a transcriptomic dataset for the Arctic collembolan, Megaphorura arctica. Although B. antarctica and M. arctica are adapted to similar environments, our analysis indicated very little overlap in expression profiles between these two arthropods. Whereas several orthologous genes showed similar expression patterns, transcriptional changes were largely species specific, indicating these polar arthropods have developed distinct transcriptional mechanisms to cope with similar desiccating conditions. © 2012, National Academy of Sciences. All rights reserved.
英文关键词	Environmental stress; Physiological ecology
语种	英语
scopus关键词	adenosine triphosphate; cytochrome c; proteasome; ubiquitin; primer DNA; Antarctica; Article; autophagy; Belgica antarctica; body water; controlled study; dehydration; down regulation; environmental stress; gene expression; insect; Megaphorura arctica; next generation sequencing; nonhuman; oxidation reduction reaction; priority journal; proton transport; RNA sequence; transcriptomics; upregulation; water loss; acclimatization; animal; arthropod; article; Chironomidae; comparative study; dehydration; gene; gene expression; genetics; metabolome; nucleotide sequence; pathophysiology; physiology; species difference; Acclimatization; Animals; Antarctic Regions; Arthropods; Base Sequence; Chironomidae; Dehydration; DNA Primers; Gene Expression; Genes, Insect; Metabolome; Species Specificity
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/158998
作者单位	Teets, N.M., Department of Entomology, Ohio State University, Columbus, OH 43210, United States; Peyton, J.T., Department of Evolution, Ecology, and Organismal Biology, Ohio State University, Columbus, OH 43210, United States; Colinet, H., Unité Mixte de Recherche, Centre National de la Recherche Scientifique 6553 Ecobio, Université de Rennes 1, Rennes Cedex, 35042, France, Earth and Life Institute, Biodiversity Research Centre (BDIV), Catholic University of Louvain, Louvain-la-Neuve, B-1348, Belgium; Renault, D., Unité Mixte de Recherche, Centre National de la Recherche Scientifique 6553 Ecobio, Université de Rennes 1, Rennes Cedex, 35042, France; Kelley, J.L., Department of Genetics, Stanford University, Stanford, CA 94305, United States; Kawarasaki, Y., Department of Zoology, Miami University, Oxford, OH 45056, United States; Lee, R.E., Jr., Department of Zoology, Miami University, Oxford, OH 45056, United States; Denlinger, D.L., Department of Entomology, Ohio State University, Columbus, OH 4321...
推荐引用方式 GB/T 7714	Teets N.M.,Peyton J.T.,Colinet H.,et al. Gene expression changes governing extreme dehydration tolerance in an Antarctic insect[J],2012,109(50).
APA	Teets N.M..,Peyton J.T..,Colinet H..,Renault D..,Kelley J.L..,...&Denlinger D.L..(2012).Gene expression changes governing extreme dehydration tolerance in an Antarctic insect.Proceedings of the National Academy of Sciences of the United States of America,109(50).
MLA	Teets N.M.,et al."Gene expression changes governing extreme dehydration tolerance in an Antarctic insect".Proceedings of the National Academy of Sciences of the United States of America 109.50(2012).