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DOI	10.1073/pnas.1701425114
	Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months
	Wakayama S.; Kamada Y.; Yamanaka K.; Kohda T.; Suzuki H.; Shimazu T.; Tada M.N.; Osada I.; Nagamatsu A.; Kamimura S.; Nagatomo H.; Mizutani E.; Ishino F.; Yano S.; Wakayama T.
发表日期	2017
ISSN	0027-8424
起始页码	5988
结束页码	5993
卷号	114 期号:23
英文摘要	If humans ever start to live permanently in space, assisted reproductive technology using preserved spermatozoa will be important for producing offspring; however, radiation on the International Space Station (ISS) is more than 100 times stronger than that on Earth, and irradiation causes DNA damage in cells and gametes. Here we examined the effect of space radiation on freeze-dried mouse spermatozoa held on the ISS for 9 mo at-95 °C, with launch and recovery at room temperature. DNA damage to the spermatozoa and male pronuclei was slightly increased, but the fertilization and birth rates were similar to those of controls. Nextgeneration sequencing showed only minor genomic differences between offspring derived from space-preserved spermatozoa and controls, and all offspring grew to adulthood and had normal fertility. Thus, we demonstrate that although space radiation can damage sperm DNA, it does not affect the production of viable offspring after at least 9 mo of storage on the ISS.
英文关键词	Fertilization; Freeze-dry; International Space Station; Preservation; Spermatozoa
语种	英语
scopus关键词	double stranded DNA; histone H2AX; messenger RNA; animal cell; animal experiment; Article; birth rate; comparative study; controlled study; cosmic radiation; double stranded DNA break; embryo; embryo transfer; epididymis; fertilization; freeze drying; gene expression; in vitro fertilization; in vitro study; intracytoplasmic sperm injection; male; male fertility; mouse; next generation sequencing; nonhuman; priority journal; progeny; pronucleus; room temperature; sperm preservation; spermatozoon; animal; DNA damage; embryo development; female; freeze drying; germ cell; infertility therapy; litter size; mortality; oocyte; physiology; procedures; radiation response; space flight; spermatozoon; Animals; DNA Damage; Embryo Transfer; Embryonic Development; Female; Freeze Drying; Germ Cells; Litter Size; Male; Mice; Oocytes; Reproductive Techniques, Assisted; Space Flight; Sperm Injections, Intracytoplasmic; Spermatozoa
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/158968
作者单位	Wakayama, S., Advanced Biotechnology Center, University of Yamanashi, Yamanashi, 400-8510, Japan; Kamada, Y., Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi, 400-8510, Japan; Yamanaka, K., Department of Experimental Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, 734-8553, Japan; Kohda, T., Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan; Suzuki, H., Department of Science and Applications, Japan Space Forum, Tsukuba, 305-8505, Japan; Shimazu, T., Department of Science and Applications, Japan Space Forum, Tsukuba, 305-8505, Japan; Tada, M.N., Japan Manned Space Systems Corporation, Tokyo, 100-0004, Japan; Osada, I., Japan Manned Space Systems Corporation, Tokyo, 100-0004, Japan; Nagamatsu, A., Japan Aerospace Exploration Agency, Tsukuba, 305-8505, Japan; Kamimura, S., Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi, 400-8510, Jap...
推荐引用方式 GB/T 7714	Wakayama S.,Kamada Y.,Yamanaka K.,et al. Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months[J],2017,114(23).
APA	Wakayama S..,Kamada Y..,Yamanaka K..,Kohda T..,Suzuki H..,...&Wakayama T..(2017).Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months.Proceedings of the National Academy of Sciences of the United States of America,114(23).
MLA	Wakayama S.,et al."Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months".Proceedings of the National Academy of Sciences of the United States of America 114.23(2017).