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DOI	10.1073/pnas.1702274114
	Nonenzymatic gluconeogenesis-like formation of fructose 1;6-bisphosphate in ice
	Messner C.B.; Driscoll P.C.; Piedrafita G.; De Volder M.F.L.; Ralser M.
发表日期	2017
ISSN	0027-8424
起始页码	7403
结束页码	7407
卷号	114 期号:28
英文摘要	The evolutionary origins of metabolism, in particular the emergence of the sugar phosphates that constitute glycolysis, the pentose phosphate pathway, and the RNA and DNA backbone, are largely unknown. In cells, a major source of glucose and the large sugar phosphates is gluconeogenesis. This ancient anabolic pathway (re-)builds carbon bonds as cleaved in glycolysis in an aldol condensation of the unstable catabolites glyceraldehyde 3-phosphate and dihydroxyacetone phosphate, forming the much more stable fructose 1,6-bisphosphate. We here report the discovery of a nonenzymatic counterpart to this reaction. The in-ice nonenzymatic aldol addition leads to the continuous accumulation of fructose 1,6-bisphosphate in a permanently frozen solution as followed over months. Moreover, the in-ice reaction is accelerated by simple amino acids, in particular glycine and lysine. Revealing that gluconeogenesis may be of nonenzymatic origin, our results shed light on how glucose anabolism could have emerged in early life forms. Furthermore, the amino acid acceleration of a key cellular anabolic reaction may indicate a link between prebiotic chemistry and the nature of the first metabolic enzymes. © 2017 National Academy of Sciences. All rights reserved.
英文关键词	Evolution; Gluconeogenesis; Metabolism; Nonenzymatic reactions; Origin of metabolism
语种	英语
scopus关键词	fructose 1,6 bisphosphate; ice; amino acid; fructose bisphosphate; fructose bisphosphate aldolase; fructose-1,6-diphosphate; glucose; sugar phosphate; addition reaction; aldol reaction; Article; biosynthesis; carbon bond; catalysis; chemical bond; controlled study; gluconeogenesis; glycolysis; nonenzymatic gluconeogenesis; pH; priority journal; reaction analysis; temperature sensitivity; chemistry; metabolism; nuclear magnetic resonance spectroscopy; pentose phosphate cycle; phosphorylation; temperature; time factor; Amino Acids; Fructose-Bisphosphate Aldolase; Fructosediphosphates; Gluconeogenesis; Glucose; Glycolysis; Hydrogen-Ion Concentration; Ice; Magnetic Resonance Spectroscopy; Pentose Phosphate Pathway; Phosphorylation; Sugar Phosphates; Temperature; Time Factors
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/158967
作者单位	Messner, C.B., Molecular Biology of Metabolism Laboratory, Francis Crick Institute, London, NW1 1AT, United Kingdom, Department of Biochemistry, Cambridge Systems Biology Centre, University of Cambridge, Cambridge, CB2 1GA, United Kingdom; Driscoll, P.C., Metabolomics Science Technology Platform, Francis Crick Institute, London, NW1 1AT, United Kingdom; Piedrafita, G., Department of Biochemistry, Cambridge Systems Biology Centre, University of Cambridge, Cambridge, CB2 1GA, United Kingdom, Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, United Kingdom; De Volder, M.F.L., Department of Engineering, Institute for Manufacturing, University of Cambridge, Cambridge, CB3 0FS, United Kingdom; Ralser, M., Molecular Biology of Metabolism Laboratory, Francis Crick Institute, London, NW1 1AT, United Kingdom, Department of Biochemistry, Cambridge Systems Biology Centre, University of Cambridge, Cambridge, CB2 1GA, United Kingdom
推荐引用方式 GB/T 7714	Messner C.B.,Driscoll P.C.,Piedrafita G.,et al. Nonenzymatic gluconeogenesis-like formation of fructose 1;6-bisphosphate in ice[J],2017,114(28).
APA	Messner C.B.,Driscoll P.C.,Piedrafita G.,De Volder M.F.L.,&Ralser M..(2017).Nonenzymatic gluconeogenesis-like formation of fructose 1;6-bisphosphate in ice.Proceedings of the National Academy of Sciences of the United States of America,114(28).
MLA	Messner C.B.,et al."Nonenzymatic gluconeogenesis-like formation of fructose 1;6-bisphosphate in ice".Proceedings of the National Academy of Sciences of the United States of America 114.28(2017).