气候变化领域集成服务门户(CCPortal): Genome-wide fitness assessment during diurnal growth reveals an expanded role of the cyanobacterial circadian clock protein KaiA

CCPortal

DOI	10.1073/pnas.1802940115
	Genome-wide fitness assessment during diurnal growth reveals an expanded role of the cyanobacterial circadian clock protein KaiA
	Welkie D.G.; Rubin B.E.; Chang Y.-G.; Diamond S.; Rifkin S.A.; LiWang A.; Golden S.S.
发表日期	2018
ISSN	0027-8424
起始页码	E7174
结束页码	E7183
卷号	115 期号:30
英文摘要	The recurrent pattern of light and darkness generated by Earth’s axial rotation has profoundly influenced the evolution of organisms, selecting for both biological mechanisms that respond acutely to environmental changes and circadian clocks that program physiology in anticipation of daily variations. The necessity to integrate environmental responsiveness and circadian programming is exemplified in photosynthetic organisms such as cyanobacteria, which depend on light-driven photochemical processes. The cyanobacterium Synechococcus elongatus PCC 7942 is an excellent model system for dissecting these entwined mechanisms. Its core circadian oscillator, consisting of three proteins, KaiA, KaiB, and KaiC, transmits time-of-day signals to clock-output proteins, which reciprocally regulate global transcription. Research performed under constant light facilitates analysis of intrinsic cycles separately from direct environmental responses but does not provide insight into how these regulatory systems are integrated during light–dark cycles. Thus, we sought to identify genes that are specifically necessary in a day–night environment. We screened a dense bar-coded transposon library in both continuous light and daily cycling conditions and compared the fitness consequences of loss of each nonessential gene in the genome. Although the clock itself is not essential for viability in light–dark cycles, the most detrimental mutations revealed by the screen were those that disrupt KaiA. The screen broadened our understanding of light–dark survival in photosynthetic organisms, identified unforeseen clock–protein interaction dynamics, and reinforced the role of the clock as a negative regulator of a nighttime metabolic program that is essential for S. elongatus to survive in the dark. © 2018 National Academy of Sciences. All Rights Reserved.
英文关键词	Circadian clock; Cyanobacteria; Diurnal physiology; Photosynthesis; Transposon sequencing
语种	英语
scopus关键词	clock protein KaiA; transcription factor CLOCK; unclassified drug; bacterial protein; circadian rhythm signaling protein; KaiA protein, cyanobacteria; Article; bacterial gene; bacterial viability; circadian rhythm; controlled study; cyanophage; gene identification; gene library; gene loss; gene mutation; genetic screening; genome-wide association study; kaiA gene; light dark cycle; metabolic regulation; molecular dynamics; nonhuman; phototrophic bacterium; priority journal; protein interaction; survival; Synechococcus elongatus; transposon; circadian rhythm; genetics; metabolism; photosynthesis; physiology; signal transduction; Synechococcus; Bacterial Proteins; Circadian Clocks; Circadian Rhythm Signaling Peptides and Proteins; Genome-Wide Association Study; Photosynthesis; Signal Transduction; Synechococcus
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/158966
作者单位	Welkie, D.G., Center for Circadian Biology, University of California, San Diego, La Jolla, CA 92093, United States; Rubin, B.E., Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, United States; Chang, Y.-G., School of Natural Sciences, University of California, Merced, CA 95343, United States; Diamond, S., Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, United States, Department of Earth and Planetary Science, University of California, Berkeley, CA 94720, United States; Rifkin, S.A., Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, United States; LiWang, A., Center for Circadian Biology, University of California, San Diego, La Jolla, CA 92093, United States, School of Natural Sciences, University of California, Merced, CA 95343, United States, Chemistry and Chemical Biology, University of California, Merced, CA 95343, United States, Quantitative and Systems Biology, Universit...
推荐引用方式 GB/T 7714	Welkie D.G.,Rubin B.E.,Chang Y.-G.,et al. Genome-wide fitness assessment during diurnal growth reveals an expanded role of the cyanobacterial circadian clock protein KaiA[J],2018,115(30).
APA	Welkie D.G..,Rubin B.E..,Chang Y.-G..,Diamond S..,Rifkin S.A..,...&Golden S.S..(2018).Genome-wide fitness assessment during diurnal growth reveals an expanded role of the cyanobacterial circadian clock protein KaiA.Proceedings of the National Academy of Sciences of the United States of America,115(30).
MLA	Welkie D.G.,et al."Genome-wide fitness assessment during diurnal growth reveals an expanded role of the cyanobacterial circadian clock protein KaiA".Proceedings of the National Academy of Sciences of the United States of America 115.30(2018).