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DOI	10.1007/s11032-009-9364-x
	Development and polymorphism of Vigna unguiculata ssp unguiculata microsatellite markers used for phylogenetic analysis in asparagus bean (Vigna unguiculata ssp sesquipedialis (L.) Verdc.)
	Xu, Pei; Wu, Xiaohua; Wang, Baogen; Liu, Yonghua; Qin, Dehui; Ehlers, Jeffery D.; Close, Timothy J.; Hu, Tingting; Lu, Zhongfu; Li, Guojing
发表日期	2010
ISSN	1380-3743
起始页码	675
结束页码	684
卷号	25 期号:4
英文摘要	Asparagus bean (V. unguiculata ssp. sesquipedialis), a specific form of cowpea (V. unguiculata L. Walp.), is cultivated as a vegetable crop throughout eastern and southern Asia for its tender long pods. Little is known about the genetic relationship between asparagus bean and the broader species, particularly the dominant ssp. unguiculata. We report here the development and transferability of simple sequence repeat (SSR) markers, over 40% of which are EST-derived, from ssp. unguiculata to asparagus bean and the use of a subset of the polymorphic markers to assess the genetic diversity of asparagus bean cultivars from diverse geographic origins across China. A total of 410 EST derived SSR (eSSR) markers and 600 SSR markers derived from cowpea genespace sequences (GSS) were developed, with a cross-subspecies transferability of 100% and 98.5%, respectively. In a recombinant inbred line population of asparagus bean, a 1:1 segregation was observed for most loci. Principal coordinate analysis (PCA) and phylogenetic clustering based on 62 alleles detected by 14 polymorphic SSR markers distinguished ssp. unguiculata and sesquipedialis into separate groups. Improved asparagus bean cultivars in China generally have a narrow genetic basis compared with landraces varieties. This suggests that asparagus bean breeding programs need to consider utilizing landrace germplasm to enhance genetic variability and ensure long-term gains from selection and reduce genetic vulnerability to pathogen/pest epidemics. Because of their transferability across subspecies, the SSR markers described in this study could be effectively employed in cross-subspecies trait introgression breeding from ssp. unguiculata to sesquipedialis.
英文关键词	Asparagus bean; Cowpea; Genetic diversity; RIL; SSR; Vigna
WOS研究方向	Agriculture ; Plant Sciences ; Genetics & Heredity
WOS类目	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture
来源期刊	MOLECULAR BREEDING
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/158662
作者单位	Li, GJ (corresponding author), Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou 310021, Zhejiang, Peoples R China.
推荐引用方式 GB/T 7714	Xu, Pei,Wu, Xiaohua,Wang, Baogen,et al. Development and polymorphism of Vigna unguiculata ssp unguiculata microsatellite markers used for phylogenetic analysis in asparagus bean (Vigna unguiculata ssp sesquipedialis (L.) Verdc.)[J],2010,25(4).
APA	Xu, Pei.,Wu, Xiaohua.,Wang, Baogen.,Liu, Yonghua.,Qin, Dehui.,...&Li, Guojing.(2010).Development and polymorphism of Vigna unguiculata ssp unguiculata microsatellite markers used for phylogenetic analysis in asparagus bean (Vigna unguiculata ssp sesquipedialis (L.) Verdc.).MOLECULAR BREEDING,25(4).
MLA	Xu, Pei,et al."Development and polymorphism of Vigna unguiculata ssp unguiculata microsatellite markers used for phylogenetic analysis in asparagus bean (Vigna unguiculata ssp sesquipedialis (L.) Verdc.)".MOLECULAR BREEDING 25.4(2010).