气候变化领域集成服务门户(CCPortal): Loss of Polycystin-1 Inhibits Bicc1 Expression during Mouse Development

CCPortal

DOI	10.1371/journal.pone.0088816
	Loss of Polycystin-1 Inhibits Bicc1 Expression during Mouse Development
	Lian, Peiwen; Li, Ao; Li, Yuan; Liu, Haichao; Liang, Dan; Hu, Bo; Lin, De; Jiang, Tang; Moeckel, Gilbert; Qin, Dahui; Wu, Guanqing
发表日期	2014
ISSN	1932-6203
卷号	9 期号:3
英文摘要	Bicc1 is a mouse homologue of Drosophila Bicaudal-C (dBic-C), which encodes an RNA-binding protein. Orthologs of dBic-C have been identified in many species, from C. elegans to humans. Bicc1-mutant mice exhibit a cystic phenotype in the kidney that is very similar to human polycystic kidney disease. Even though many studies have explored the gene characteristics and its functions in multiple species, the developmental profile of the Bicc1 gene product (Bicc1) in mammal has not yet been completely characterized. To this end, we generated a polyclonal antibody against Bicc1 and examined its spatial and temporal expression patterns during mouse embryogenesis and organogenesis. Our results demonstrated that Bicc1 starts to be expressed in the neural tube as early as embryonic day (E) 8.5 and is widely expressed in epithelial derivatives including the gut and hepatic cells at E10.5, and the pulmonary bronchi at E11.5. In mouse kidney development, Bicc1 appears in the early ureteric bud and mesonephric tubules at E11.5 and is also expressed in the metanephros at the same stage. During postnatal kidney development, Bicc1 expression gradually expands from the cortical to the medullary and papillary regions, and it is highly expressed in the proximal tubules. In addition, we discovered that loss of the Pkd1 gene product, polycystin-1 (PC1), whose mutation causes human autosomal dominant polycystic kidney disease (ADPKD), downregulates Bicc1 expression in vitro and in vivo. Our findings demonstrate that Bicc1 is developmentally regulated and reveal a new molecular link between Bicc1 and Pkd1.
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
来源期刊	PLOS ONE
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/158596
作者单位	Wu, GQ (corresponding author), Chinese Acad Med Sci, Canc Hosp & Inst, State Key Lab Mol Oncol, Div Translat Canc Res & Therapy, Beijing 100730, Peoples R China.
推荐引用方式 GB/T 7714	Lian, Peiwen,Li, Ao,Li, Yuan,et al. Loss of Polycystin-1 Inhibits Bicc1 Expression during Mouse Development[J],2014,9(3).
APA	Lian, Peiwen.,Li, Ao.,Li, Yuan.,Liu, Haichao.,Liang, Dan.,...&Wu, Guanqing.(2014).Loss of Polycystin-1 Inhibits Bicc1 Expression during Mouse Development.PLOS ONE,9(3).
MLA	Lian, Peiwen,et al."Loss of Polycystin-1 Inhibits Bicc1 Expression during Mouse Development".PLOS ONE 9.3(2014).