气候变化领域集成服务门户(CCPortal): Bioconcentration, metabolism and effects of diphenhydramine on behavioral and biochemical markers in crucian carp (Carassius auratus)

CCPortal

DOI	10.1016/j.scitotenv.2015.11.132
	Bioconcentration, metabolism and effects of diphenhydramine on behavioral and biochemical markers in crucian carp (Carassius auratus)
	Xie, Zhengxin; Lu, Guanghua; Hou, Kangkang; Qin, Donghong; Yan, Zhenhua; Chen, Wei
发表日期	2016
ISSN	0048-9697
起始页码	400
结束页码	409
卷号	544
英文摘要	Diphenhydramine (DPH), an antihistamine used to alleviate human allergies, is widespread in aquatic environments. However, little is known about the biochemical and behavioral effects of DPH on non-target aquatic animals. In the present study, the tissue distribution, bioconcentration, metabolism, biochemical and behavioral effects were investigated in crucian carp (Carassius auratus) exposed to various concentrations of DPH (0.84, 4.23, 21.7 and 112 mu g L-1) for 7 d. DPH can accumulate in crucian carp, and high concentrations have been observed in the liver and brain with maximum bioconcentration factors of 148 and 81.6, respectively. A portion of the absorbed DPH was metabolized by the crucian carp to N-demethyl DPH and N,N-didemethyl DPH via N-demethylation. Direct fluorimetric assay was employed to assess metabolic activity, while oxidative stress and neurotransmission biomarkers were determined by Diagnostic Reagent Kits. DPH was found to increase hepatic 7-ethoxyresorufin O-deethylase activity in crucian carp with maximal induction of 119%. Concerning the oxidative stress status, DPH significantly inhibited superoxide dismutase (SOD, 37-58%) and glutathione S-transferase (GST, 43-65%) activities and led to a significant increase in malondialdehyde (MDA, 67-140%) levels and catalase (CAT, 38-143%) and glutathione peroxidase (GPx, 39-189%) activities in fish liver. Brain acetylcholinesterase activity was also induced in DPH-exposed crucian carp with maximal induction of 174%. In addition, shoaling was significantly enhanced, while swimming activity and feeding rates were markedly suppressed at DPH concentrations equal to or higher than 21.7 mu g L-1. Furthermore, significant correlations were found between oxidative stress biomarkers (SOD, CAT, GPx, GST and MDA) and behavioral parameters. Collectively, our results confirmed that DPH can accumulate and be metabolized in fish and exert a negative effect at different levels of biological organization. (C) 2015 Elsevier B.V. All rights reserved.
英文关键词	Diphenhydramine; Bioconcentration; Metabolism; Antioxidant enzymes; Behavior
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
来源期刊	SCIENCE OF THE TOTAL ENVIRONMENT
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/158556
作者单位	Lu, GH (corresponding author), Hohai Univ, Coll Environm, Minist Educ, Key Lab Integrated Regulat & Resources Dev Shallo, Nanjing 210098, Jiangsu, Peoples R China.
推荐引用方式 GB/T 7714	Xie, Zhengxin,Lu, Guanghua,Hou, Kangkang,et al. Bioconcentration, metabolism and effects of diphenhydramine on behavioral and biochemical markers in crucian carp (Carassius auratus)[J],2016,544.
APA	Xie, Zhengxin,Lu, Guanghua,Hou, Kangkang,Qin, Donghong,Yan, Zhenhua,&Chen, Wei.(2016).Bioconcentration, metabolism and effects of diphenhydramine on behavioral and biochemical markers in crucian carp (Carassius auratus).SCIENCE OF THE TOTAL ENVIRONMENT,544.
MLA	Xie, Zhengxin,et al."Bioconcentration, metabolism and effects of diphenhydramine on behavioral and biochemical markers in crucian carp (Carassius auratus)".SCIENCE OF THE TOTAL ENVIRONMENT 544(2016).