气候变化领域集成服务门户(CCPortal): Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs

CCPortal

DOI	10.3791/58242
	Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs
	Wang, Lingping; Wu, Xinyi; Li, Guojing; Wu, Xiaohua; Qin, Dehui; Tao, Ye; Xu, Pei
发表日期	2018
ISSN	1940-087X
期号	141
英文摘要	MicroRNAs (miRNAs) are endogenous small non-coding RNAs of about 20 - 24 nt, known to play important roles in plant development and adaptation. There is an accumulating evidence showing that the expressions of certain miRNAs are altered when grafting, an agricultural practice commonly used by farmers to improve crop tolerance to biotic and abiotic stresses. Bottle gourd is an inherently climate-resilient crop compared to many other major cucurbits, including watermelon, rendering it one of the most widely used rootstocks for the latter. The recent advancement of high-throughput sequencing technologies has provided great opportunities to investigate cold-responsive miRNAs and their contributions to heterograft advantages; yet, adequate experimental procedures are a prerequisite for this purpose. Here, we present a detailed protocol for efficiently generating homo- and heterografts between the cold-susceptible watermelon and the cold-tolerant bottle gourd, in addition to methods of tissue sampling, data generation, and data analysis. The presented methods are also useful for other plant-grafting systems, to interrogate miRNA regulations under various environmental stresses, such as heat, drought, and salinity.
英文关键词	Environmental Sciences; Issue 141; bottle gourd; cold stress; differential expression; graft; miRNA; watermelon
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
来源期刊	JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/158512
作者单位	Xu, P (corresponding author), Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou, Zhejiang, Peoples R China.; Xu, P (corresponding author), Zhejiang Acad Agr Sci, State Key Lab Breeding Base Sustainable Control P, Hangzhou, Zhejiang, Peoples R China.
推荐引用方式 GB/T 7714	Wang, Lingping,Wu, Xinyi,Li, Guojing,et al. Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs[J],2018(141).
APA	Wang, Lingping.,Wu, Xinyi.,Li, Guojing.,Wu, Xiaohua.,Qin, Dehui.,...&Xu, Pei.(2018).Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs.JOVE-JOURNAL OF VISUALIZED EXPERIMENTS(141).
MLA	Wang, Lingping,et al."Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs".JOVE-JOURNAL OF VISUALIZED EXPERIMENTS .141(2018).