气候变化领域集成服务门户(CCPortal): Single and combined effects of selected haloacetonitriles in a human-derived Check for hepatoma line

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DOI	10.1016/j.ecoenv.2018.07.104
	Single and combined effects of selected haloacetonitriles in a human-derived Check for hepatoma line
	Lu, Guanghua; Qin, Donghong; Wang, Yonghua; Liu, Jianchao; Chen, Wei
发表日期	2018
ISSN	0147-6513
起始页码	417
结束页码	426
卷号	163
英文摘要	Haloacetonitriles (HANs) are nitrogenous disinfection byproducts (N-DBPs) detected in drinking water that have high toxicity and are a high risk to human health. The cytotoxicity and genotoxicity as well as the oxidative stress of five HANs, namely chloroacetonitrile (CAN), dichloroacetonitrile (DCAN), trichloroacetonitrile (TCAN), bromoacetonitrile (BAN), and dibromoacetonitrile (DBAN) on a hepatoma cell line (HepG2) were determined by single, binary or ternary exposure. The median effective concentrations, based on cell viability, ranged from 0.8360 mg/L for BAN to 256.9 mg/L for DCAN, with a cytotoxicity order of BAN > DBAN > CAN > TCAN > DCAN. The lowest observed effective concentrations regarding DNA damage were 0.01 mg/L for CAN and DCAN, 0.1 mg/L for DBAN and TCAN, and 1 mg/L for BAN. The DNA damage induced by CAN, DCAN and TCAN was repaired to about 80% in 30 min, and when induced by BAN and DBAN, it was repaired completely in 60 min. The intracellular reactive oxygen species (ROS) levels were significantly increased by the five HANs, and bromo-acetonitrile produced a stronger oxidative stress than chloro-acetonitrile. Co-exposure of DCAN, TCAN and DBAN significantly inhibited cell viability, induced DNA damage and facilitated ROS generation in HepG2 cells. However, the interactive effects were inconsistent for the different endpoints, which seemed to be antagonism for cell viability but synergy for ROS generation.
英文关键词	Haloacetonitriles; Cytotoxicity; DNA damage; ROS; Combination toxicity; CA and IA models
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
WOS类目	Environmental Sciences ; Toxicology
来源期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/158511
作者单位	Lu, GH (corresponding author), Tibet Agr & Anim Husb Univ, Water Conservancy Project & Civil Engn Coll, Linzhi 860000, Peoples R China.
推荐引用方式 GB/T 7714	Lu, Guanghua,Qin, Donghong,Wang, Yonghua,et al. Single and combined effects of selected haloacetonitriles in a human-derived Check for hepatoma line[J],2018,163.
APA	Lu, Guanghua,Qin, Donghong,Wang, Yonghua,Liu, Jianchao,&Chen, Wei.(2018).Single and combined effects of selected haloacetonitriles in a human-derived Check for hepatoma line.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,163.
MLA	Lu, Guanghua,et al."Single and combined effects of selected haloacetonitriles in a human-derived Check for hepatoma line".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 163(2018).