气候变化领域集成服务门户(CCPortal): Role of AURKA in the hypothalamus–pituitary–testicular axis in Tibetan sheep from Tianzhu

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DOI	10.1016/j.ygcen.2020.113617
	Role of AURKA in the hypothalamus–pituitary–testicular axis in Tibetan sheep from Tianzhu
	Wang Y.; Yang Y.; Gan Z.; Zhao C.; Lv C.; Zhang Y.; Zhao X.
发表日期	2021
ISSN	166480
卷号	300
英文摘要	The mitosis-associated protein aurora kinase A (AURKA) regulates the maturation of germ cells. We have previously reported using transcriptome analysis that AURKA is expressed in yak testes. Although Tibetan sheep possess an immense economic value, their reproductive rate is low. Herein, the expression and functions of AURKA in the hypothalamus–pituitary–testicular (HPT) axis in Tibetan sheep from Tianzhu were investigated. The cDNA sequence of sheep AURKA was cloned and bioinformatics techniques were used to predict its structure. Tissue expression of AURKA was determined by qPCR, immunoblotting, immunostaining, and immunohistochemistry. The AURKA coding sequence was found to be 1218 bp in length, encoding a 405-amino acid polypeptide chain. Furthermore, the highest sequence similarity of AURKA with the corresponding sequence in other species was seen in goat and cattle; the least degree of similarity was seen in the domestic cat. In addition, AURKA expression was elevated in the testes compared to that in the hypothalamus and pituitary (p < 0.01). Moreover, AURKA was mainly localized in the hypothalamic paraventricular nucleus (magnocellular), chromophobe cells of the pituitary, and spermatogenic cells of the testis. These results indicated that AURKA might participate in sheep reproductive regulation, thus providing a reference for the study of AURKA function in the reproductive process of Tibetan sheep from Tianzhu. © 2020
英文关键词	AURKA; Hypothalamus–pituitary–testicular axis; Sertoli cells; Tibetan sheep from Tianzhu
语种	英语
scopus关键词	General and Comparative Endocrinology
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/158336
作者单位	College of Veterinary Medicine; Gansu Agricultural University; Lanzhou; China; College of Life Science and Technology; Gansu Agricultural University; Lanzhou; China
推荐引用方式 GB/T 7714	Wang Y.,Yang Y.,Gan Z.,等. Role of AURKA in the hypothalamus–pituitary–testicular axis in Tibetan sheep from Tianzhu[J],2021,300.
APA	Wang Y..,Yang Y..,Gan Z..,Zhao C..,Lv C..,...&Zhao X..(2021).Role of AURKA in the hypothalamus–pituitary–testicular axis in Tibetan sheep from Tianzhu.,300.
MLA	Wang Y.,et al."Role of AURKA in the hypothalamus–pituitary–testicular axis in Tibetan sheep from Tianzhu".300(2021).