气候变化领域集成服务门户(CCPortal): Targeted isolation of 1;1-diphenyl-2-picrylhydrazyl inhibitors from Saxifraga atrata using medium- and high- pressure liquid chromatography combined with online high performance liquid chromatography

CCPortal

DOI	10.1016/j.chroma.2020.461690
	Targeted isolation of 1;1-diphenyl-2-picrylhydrazyl inhibitors from Saxifraga atrata using medium- and high- pressure liquid chromatography combined with online high performance liquid chromatography–1;1-diphenyl-2- picrylhydrazyl detection
	Dawa Y.; Du Y.; Wang Q.; Chen C.; Zou D.; Qi D.; Ma J.; Dang J.
发表日期	2021
ISSN	219673
卷号	1635
英文摘要	Traditional Tibetan medicine (TTM) is a valuable source of novel therapeutic lead molecules inspired by natural products (NPs). The health benefits of Saxifraga atrata are well documented in TTM, but reports on its chemical composition are limited, most likely due to the complicated purification process. Herein, target separation and identification of 4 main radical scavenging compounds from the methanolic extract of S. atrata was were performed using medium- and high-pressure liquid chromatography coupled with online HPLC-DPPH detection. The sample was pretreated using medium pressure liquid chromatography with MCI GELⓇ CHP20P styrene-divinylbenzene beads as a stationary phase, yielding 1.4 g of the target DPPH inhibitors (Fr4, 11.9% recovery). The compounds were further purified and isolated using HPLC on RP-C18 (ReproSil-Pur C18 AQ) followed by HILIC (Click XIon) column separation, resulting in 2.8 mg of fraction Fr4-1-1, 6.8 mg of fraction Fr4-2, 244.9 mg of the Fr4-3-1 sample, and 38.3 mg of Fr4-4-1. The structure and purity of the target compounds were determined, and four compounds (ethyl gallate, 11-O-galloylbergenin, rutin and isoquercitrin) were isolated with >95% purity. The developed methodology is efficient for targeted isolation of high-purity radical scavengers from NP extracts and could be used for rapid identification and isolation of DPPH inhibitors from various NPs. © 2020
英文关键词	DPPH inhibitors; High-pressure liquid chromatography; Medium-pressure liquid chromatography; Online HPLC-DPPH assay; Saxifraga atrata
语种	英语
scopus关键词	Journal of Chromatography A
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/158328
作者单位	Qinghai Provincial Key Laboratory of Tibet Plateau Biodiversity Formation Mechanism and Comprehensive Utilization; College of Life Sciences; Qinghai Normal University; Xining; 810008; China; Qinghai Provincial Key Laboratory of Tibetan Medicine Research; Key Laboratory of Tibetan Medicine Research; Northwest Institute of Plateau Biology; Chinese Academy of Sciences; Xining; Qinghai; China; College of Pharmacy; Qinghai Nationalities University; Xining; Qinghai; China
推荐引用方式 GB/T 7714	Dawa Y.,Du Y.,Wang Q.,等. Targeted isolation of 1;1-diphenyl-2-picrylhydrazyl inhibitors from Saxifraga atrata using medium- and high- pressure liquid chromatography combined with online high performance liquid chromatography–1;1-diphenyl-2- picrylhydrazyl detection[J],2021,1635.
APA	Dawa Y..,Du Y..,Wang Q..,Chen C..,Zou D..,...&Dang J..(2021).Targeted isolation of 1;1-diphenyl-2-picrylhydrazyl inhibitors from Saxifraga atrata using medium- and high- pressure liquid chromatography combined with online high performance liquid chromatography–1;1-diphenyl-2- picrylhydrazyl detection.,1635.
MLA	Dawa Y.,et al."Targeted isolation of 1;1-diphenyl-2-picrylhydrazyl inhibitors from Saxifraga atrata using medium- and high- pressure liquid chromatography combined with online high performance liquid chromatography–1;1-diphenyl-2- picrylhydrazyl detection".1635(2021).