气候变化领域集成服务门户(CCPortal): Neuroprotection against cerebral ischemia/reperfusion by dietary phytochemical extracts from Tibetan turnip (Brassica rapa L.)

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DOI	10.1016/j.jep.2020.113410
	Neuroprotection against cerebral ischemia/reperfusion by dietary phytochemical extracts from Tibetan turnip (Brassica rapa L.)
	Hua H.; Zhang W.; Li J.; Li J.; Liu C.; Guo Y.; Cheng Y.; Pi F.; Xie Y.; Yao W.; Gao Y.; Qian H.
发表日期	2021
ISSN	3788741
卷号	265
英文摘要	Ethnopharmacological relevance: The Tibetan turnip (Brassica rapa L.) has a wide array of medicine properties including heat-clearing, detoxifying and anti-hypoxia as listed in the famous centuries-old Tibetan medicine classic “The Four Medical Tantras”. Evidence-based medicine also indicated the anti-hypoxic effect of turnips, suggesting a potential link to neuroprotective effect on ischemic stroke. This thereby enables turnips to serve as a novel nontoxic agent in related treatment. Aim of the study: This study aimed to investigate the neuroprotective effect and elucidate the mechanism of aqueous extract of turnip (AET) on cerebral ischemia/reperfusion. Materials and methods: The experimental models of cerebral ischemia included transient middle cerebral artery occlusion/reperfusion (MCAO) in C57BL/6J mice and oxygen-glucose deprivation/reoxygenation (OGD/R) in HT-22 cells. Long-term effect of AET on infarct volume was evaluated by microtubule-associated protein 2 (MAP2) immunofluorescence 28 days after MCAO, and on neurofunctional outcomes determined by rotarod, grid walking, and cylinder tests in the meantime. Efficacy of AET was determined by the cell viability, the release of lactate dehydrogenase (LDH) and reactive oxygen species (ROS) in neurons. The underlying mechanism of AET rescued OGD/R cells were characterized by PI3K, Akt and mTOR expressions, which were further used to validate AET's role in the pathway. Results: AET can reduce cerebral infarct volume and ameliorate behavioral deficits of MCAO/R mice dose-dependently. In vitro experiment further demonstrated that suitable concentrations of AET inhibited ROS, LDH production and restored mitochondrial expression induced by OGD/R. AET pretreatment can reverse the OGD/R-induced decreased level of phosphorylation of PI3K, Akt, mTOR, whereas this effect was blocked in the LY294002 (PI3K inhibitor) treatment group. Conclusions: AET improved the survival of OGD/R-injured HT-22 cells by activating the PI3K/Akt/mTOR pathway. Based on the results above, aqueous extract of turnip has a protective effect on focal cerebral ischemic injury. © 2020 Elsevier B.V.
英文关键词	Brassica rapa L.; Cerebral ischemia/reperfusion; Neuroprotection; PI3K/AkT/mTOR
语种	英语
scopus关键词	Journal of Ethnopharmacology
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/158313
作者单位	Department of School of Food Science and Technology; Jiangnan University; Wuxi; 214122; China; State Key Laboratory of Medical Neurobiology; MOE Frontiers Center for Brain Science; Institutes of Brain Science; Fudan University; Shanghai; 200032; China
推荐引用方式 GB/T 7714	Hua H.,Zhang W.,Li J.,et al. Neuroprotection against cerebral ischemia/reperfusion by dietary phytochemical extracts from Tibetan turnip (Brassica rapa L.)[J],2021,265.
APA	Hua H..,Zhang W..,Li J..,Li J..,Liu C..,...&Qian H..(2021).Neuroprotection against cerebral ischemia/reperfusion by dietary phytochemical extracts from Tibetan turnip (Brassica rapa L.).,265.
MLA	Hua H.,et al."Neuroprotection against cerebral ischemia/reperfusion by dietary phytochemical extracts from Tibetan turnip (Brassica rapa L.)".265(2021).