气候变化领域集成服务门户(CCPortal): Horizontal and vertical gene transfer drive sediment antibiotic resistome in an urban lagoon system

CCPortal

DOI	10.1016/j.jes.2020.09.004
	Horizontal and vertical gene transfer drive sediment antibiotic resistome in an urban lagoon system
	Wang H.; Hou L.; Liu Y.; Liu K.; Zhang L.; Huang F.; Wang L.; Rashid A.; Hu A.; Yu C.
发表日期	2021
ISSN	10010742
起始页码	11
结束页码	23
卷号	102
英文摘要	Rapid urbanization has resulted in pervasive occurrence of antibiotic resistance genes (ARGs) in urban aquatic ecosystems. However, limited information is available concerning the ARG profiles and the forces responsible for their assembly in urban landscape lagoon systems. Here, we employed high-throughput quantitative PCR (HT-qPCR) to characterize the spatial variations of ARGs in surface and core sediments of Yundang Lagoon, China. The results indicated that the average richness and absolute abundance of ARGs were 11 and 53 times higher in the lagoon sediments as compared to pristine reference Tibetan lake sediments, highlighting the role of anthropogenic activities in ARG pollution. Co-occurrence network analysis indicated that various anaerobic prokaryotic genera belonging to Alpha-, Deltaproteobacteria, Bacteroidetes, Euryarchaeota, Firmicutes and Synergistetes were the potential hosts of ARGs. The partial least squares-path modeling (PLS-PM) analysis revealed positive and negative indirect effects of physicochemical factors and heavy metals on the lagoon ARG profiles, via biotic factors, respectively. The horizontal (mediated by mobile genetic elements) and vertical (mediated by prokaryotic communities) gene transfer may directly contribute the most to drive the abundance and composition of ARGs, respectively. Furthermore, the neutral community model demonstrated that the assembly of sediment ARG communities was jointly governed by deterministic and stochastic processes. Overall, this study provides novel insights into the diversity and distribution of ARGs in the benthic habitat of urban lagoon systems and underlying mechanisms for the spread and proliferation of ARGs. © 2020
英文关键词	Antibiotic resistance genes; Assembly mechanisms; Heavy metals; High-throughput quantitative PCR; Horizontal and vertical gene transfer; Urban landscape lagoon
语种	英语
scopus关键词	Journal of Environmental Sciences (China)
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/158262
作者单位	CAS Key Laboratory of Urban Pollutant Conversion; Institute of Urban Environment; Chinese Academy of Sciences; Xiamen; 361021; China; University of Chinese Academy of Sciences; Beijing; 100049; China; Department of Chemistry; SUNY College of Environmental Science and Forestry; Syracuse; NY 13210; United States; Key Laboratory of Tibetan Environment Changes and Land Surface Processes; Institute of Tibetan Plateau Research; Chinese Academy of Sciences; Beijing; 100101; China; Key Laboratory of Urban Environment and Health; Institute of Urban Environment; Chinese Academy of Sciences; Xiamen; 361021; China; Nuclear Institute for Food and Agriculture; Tarnab; Peshawar 25000; Pakistan
推荐引用方式 GB/T 7714	Wang H.,Hou L.,Liu Y.,et al. Horizontal and vertical gene transfer drive sediment antibiotic resistome in an urban lagoon system[J]. 中国科学院青藏高原研究所,2021,102.
APA	Wang H..,Hou L..,Liu Y..,Liu K..,Zhang L..,...&Yu C..(2021).Horizontal and vertical gene transfer drive sediment antibiotic resistome in an urban lagoon system.,102.
MLA	Wang H.,et al."Horizontal and vertical gene transfer drive sediment antibiotic resistome in an urban lagoon system".102(2021).