气候变化领域集成服务门户(CCPortal): Meiofaunal diversity in the Atlantic Forest soil: A quest for nematodes in a native reserve using eukaryotic metabarcoding analysis

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DOI	10.1016/j.foreco.2019.117591
	Meiofaunal diversity in the Atlantic Forest soil: A quest for nematodes in a native reserve using eukaryotic metabarcoding analysis
	Müller C.A.; de Mattos Pereira L.; Lopes C.; Cares J.; dos Anjos Borges L.G.; Giongo A.; Graeff-Teixeira C.; Loureiro Morassutti A.
发表日期	2019
ISSN	0378-1127
卷号	453
英文摘要	The assessment of environmental communities may lead to the identification of the novel or not-yet-classified organisms. Few studies have focused on the meiofauna of poorly known habitats such as native soils in subtropical forests. We surveyed eukaryotic communities inhabiting soil in an unexplored area of a native Atlantic Forest in southern Brazil using high-throughput sequencing. We analyzed a total of 281,400 sequences using V4 and V9 hypervariable regions of 18S rRNA gene. Opisthokonta was the most abundant supergroup, with Fungi and Metazoa representing an average of 40.6% and 15.8% of the sequences, respectively. Among the metazoan, Nematoda was the second most abundant phylum (4.8%). Isolation and morphological assessment 1745 specimens of nematodes, classified by functional feeding groups as plant parasites (35%), bacterial feeders (30%), omnivores (7%) and predators (5%). Specimens belonged to the class Chromadorea (89.6%) and Enoplea (10.4%).Approximately 65% of them could be morphologically classified only to order level. Results identified a considerable number of newly or still uncharacterized nematodes in soil biota in Atlantic Forest soils. Furthermore, this is the first study in this subtropical area to show that both high throughput sequencing and morphology can give complementary indications of the diversity of nematodes in soil samples. © 2019 Elsevier B.V.
英文关键词	Functional feeding groups; Morphology identification; Nematode; Opisthokonta; SAR
语种	英语
scopus关键词	Feeding; Forestry; Morphology; RNA; Tropics; Atlantic forest; Functional feeding groups; High-throughput sequencing; Morphology identification; Nematode; Opisthokonta; Subtropical area; Subtropical forests; Soils; biodiversity; eukaryote; feeding ground; forest soil; fungus; genetic analysis; identification method; meiofauna; metazoan; morphology; native species; nematode; soil biota; subtropical region; synthetic aperture radar; Anatomy; Feeding; Forestry; Nucleic Acids; Sequences; Soil; Tropics; Worms; Atlantic Forest; Brazil; Bacteria (microorganisms); Chromadorea; Enoplea; Eukaryota; Fungi; Fungi/Metazoa group; Metazoa; Nematoda
来源期刊	Forest Ecology and Management
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/155744
作者单位	Laboratório de Biologia Parasitária, Escola de Ciências, Laboratório de Parasitologia Molecular, Instituto de Pesquisas Biomédicas, Pontifícia Universidade do Rio Grande do Sul (PUCRS), Av. Ipiranga 6681, Porto Alegre, RS 90690-900, Brazil; Departamento de Fitopatologia, Instituto de Ciências Biológicas, Universidade de Brasília. Campus Universitário Darcy Ribeiro, Asa Norte, Brasília, DF 70910-900, Brazil; Laboratório de Geobiologia, Instituto do Petróleo e Recursos Naturais, Pontifícia Universidade do Rio Grande do Sul (PUCRS), Av. Ipiranga 6681, Porto Alegre, RS 90690-900, Brazil; Faculdade de Teologia e Ciências (FATEC), Rua José Sanches Peres 3040, Votuporanga, SP 5501-210, Brazil
推荐引用方式 GB/T 7714	Müller C.A.,de Mattos Pereira L.,Lopes C.,et al. Meiofaunal diversity in the Atlantic Forest soil: A quest for nematodes in a native reserve using eukaryotic metabarcoding analysis[J],2019,453.
APA	Müller C.A..,de Mattos Pereira L..,Lopes C..,Cares J..,dos Anjos Borges L.G..,...&Loureiro Morassutti A..(2019).Meiofaunal diversity in the Atlantic Forest soil: A quest for nematodes in a native reserve using eukaryotic metabarcoding analysis.Forest Ecology and Management,453.
MLA	Müller C.A.,et al."Meiofaunal diversity in the Atlantic Forest soil: A quest for nematodes in a native reserve using eukaryotic metabarcoding analysis".Forest Ecology and Management 453(2019).