气候变化领域集成服务门户(CCPortal): Seed osmopriming with PEG solutions in seeds of three infraspecific taxa of Pinus nigra: Impacts on germination, mitosis and nuclear DNA

CCPortal

DOI	10.1016/j.foreco.2019.117739
	Seed osmopriming with PEG solutions in seeds of three infraspecific taxa of Pinus nigra: Impacts on germination, mitosis and nuclear DNA
	Carvalho A.; Gaivão I.; Lima-Brito J.
发表日期	2020
ISSN	0378-1127
卷号	456
英文摘要	A phenological characterisation of the Portuguese Pinus nigra stands performed in the 1980s considered them as belonging to subspecies laricio, salzmanni and nigra. Recently, six allochthonous P. nigra populations that represent the actual species distribution in Portugal were molecularly characterised and showed high genetic similarity to subsp. laricio vars. corsicana and calabrica, and genetic distance to subsp. salzmanni. P. nigra has a hard natural regeneration, and this problem will be enhanced under the more severe and frequent drought episodes projected to Europe. In this work, seeds of the P. nigra infraspecific taxa previously assigned to Portugal were osmoprimed with 10% and 20% polyethylene glycol (PEG) solutions to mimic water stress, and to analyse its impacts on germination, mitosis, and nuclear DNA of young needles. Hydroprimed seeds and resulting plantlets were used as controls. The osmotic stress imposed to the seeds delayed the germination in most of the cases, induced cell cycle anomalies, and caused DNA damage in needles. These impacts were more pronounced in subsp. laricio var. corsicana. The detection of osmotic stress effects from germination to the juvenile plantlet stage suggested a stress memory transmission between plant tissues. The 20% PEG osmopriming performed in var. corsicana seeds was lethal. Globally, this preliminary work suggested a differential water stress tolerance among the three P. nigra infraspecific taxa that should be exploited further, and provided probable explanations for their reduced natural regeneration and early mortality of pine seedlings that compromise afforestation/reforestation programs in Portugal and abroad. © 2019 Elsevier B.V.
英文关键词	Cytogenetics; European Black Pine; Germination; Mitosis; Osmopriming; Single cell gel electrophoresis
语种	英语
scopus关键词	Cell culture; Cultivation; DNA; Electrophoresis; Needles; Osmosis; Reforestation; Value engineering; Black pines; Cytogenetics; Germination; Mitosis; Osmopriming; Single cell gel electrophoresis; Seed; coniferous forest; cytogenetics; DNA; ecological impact; electrokinesis; germination; juvenile; performance assessment; phenology; seed; spatial distribution; Cultivation; Electrophoresis; Needles; Nucleic Acids; Osmosis; Reforestation; Portugal; Pinus nigra
来源期刊	Forest Ecology and Management
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/155611
作者单位	Biosystems & Integrative Sciences Institute, Faculty of Sciences, University of Lisboa, Portugal; Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tras-os-Montes and Alto Douro, Quinta de Prados, Vila Real, 5000-801, Portugal; Animal and Veterinary Research Centre (CECAV), University of Tras-os-Montes and Alto Douro, Quinta de Prados, Vila Real, 5000-801, Portugal; Department of Genetics and Biotechnology (DGB), University of Tras-os-Montes and Alto Douro, Quinta de Prados, Vila Real, 5000-801, Portugal
推荐引用方式 GB/T 7714	Carvalho A.,Gaivão I.,Lima-Brito J.. Seed osmopriming with PEG solutions in seeds of three infraspecific taxa of Pinus nigra: Impacts on germination, mitosis and nuclear DNA[J],2020,456.
APA	Carvalho A.,Gaivão I.,&Lima-Brito J..(2020).Seed osmopriming with PEG solutions in seeds of three infraspecific taxa of Pinus nigra: Impacts on germination, mitosis and nuclear DNA.Forest Ecology and Management,456.
MLA	Carvalho A.,et al."Seed osmopriming with PEG solutions in seeds of three infraspecific taxa of Pinus nigra: Impacts on germination, mitosis and nuclear DNA".Forest Ecology and Management 456(2020).