气候变化领域集成服务门户(CCPortal): Advances in bone preservation: Identifying possible collagen preservation using sulfur speciation mapping

CCPortal

DOI	10.1016/j.palaeo.2019.01.030
	Advances in bone preservation: Identifying possible collagen preservation using sulfur speciation mapping
	Anné J.; Edwards N.P.; Brigidi F.; Gueriau P.; Harvey V.L.; Geraki K.; Slimak L.; Buckley M.; Wogelius R.A.
发表日期	2019
ISSN	0031-0182
起始页码	181
结束页码	187
卷号	520
英文摘要	The complexity and heterogeneity of bone chemistry makes it difficult to discern information on physiological and taphonomic processes stored within the bone matrix. Analysis of archaeological and palaeontological bone becomes more difficult because in many cases the most pivotal specimens are too scientifically valuable for destructive analysis. This problem is further escalated by the fact that the heterogeneity of the bone may cause small “pockets” of preservation that can be missed during sampling. Therefore, a non-destructive technique that can spatially resolve such heterogeneity within the bone is needed. Here we use microfocus, non-destructive synchrotron-based X-Ray Fluorescence (XRF) imaging and X-ray Absorption Spectroscopy (XAS) to map the organic constituents within extant and fossil bovid bones. XAS analysis of sulfur allowed organic sulfur (within collagen as methionine) to be distinguished from inorganic sulfate (within bone apatite). Mapping and quantification of organic sulfur within the samples were made by setting the beam to the methionine resonance, allowing for the detection, distribution and quantification of collagen present by using organic sulfur as an internal marker. Results show organic sulfur to be distributed in small “pockets” throughout the bone matrix in both extant and fossil specimens. Significant loss of organic sulfur was seen in specimens between 100 ka and 650 ka with little organic sulfur preservation persisting after this date. Comparison of residual organic sulfur concentrations as a function of sample age revealed a second order rate law for organic sulfur oxidation (k ≈ 1 × 10 −5 y −1 ) within bone. These results show that non-destructive, synchrotron-based XRF mapping of organic sulfur is a useful tool for not only calculating rates of collagen degradation through time, but also identifying areas of potential collagen preservation for other paleobiological applications such as proteomics and stable isotope analyses. © 2019 Elsevier B.V.
英文关键词	Collagen degradation; Sulfur species mapping
语种	英语
scopus关键词	archaeology; bone; collagen; fossil record; identification method; mapping method; paleobiology; paleontology; speciation (chemistry); sulfur; taphonomy; type specimen; Bovidae
来源期刊	Palaeogeography, Palaeoclimatology, Palaeoecology
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/151023
作者单位	University of Manchester, Interdisciplinary Centre for Ancient Life, School of Earth and Environmental Sciences, Manchester, M13 9PL, United Kingdom; The Children's Museum of Indianapolis, Indianapolis, IN 42608, United States; SLAC National Accelerator Laboratory, Menlo Park, CA 94025, United States; Elettra-Sincrotrone Trieste, S.S. 14 Km 163.5 in Area Science Park, 34149, Basovizza, Trieste, Italy; IPANEMA, CNRS, Ministère de la Culture, UVSQ, USR3461, Université Paris-Saclay, Gif-sur-Yvette, 91192, France; Diamond Light Source, Didcot, OX11 0DE, United Kingdom; CNRS, UMR 5608, TRACES, Université Toulouse- Jean Jaures, Mason de la Recherché Bât 26, 5 allé Antonio Machado, Toulouse, 31058, France
推荐引用方式 GB/T 7714	Anné J.,Edwards N.P.,Brigidi F.,et al. Advances in bone preservation: Identifying possible collagen preservation using sulfur speciation mapping[J],2019,520.
APA	Anné J..,Edwards N.P..,Brigidi F..,Gueriau P..,Harvey V.L..,...&Wogelius R.A..(2019).Advances in bone preservation: Identifying possible collagen preservation using sulfur speciation mapping.Palaeogeography, Palaeoclimatology, Palaeoecology,520.
MLA	Anné J.,et al."Advances in bone preservation: Identifying possible collagen preservation using sulfur speciation mapping".Palaeogeography, Palaeoclimatology, Palaeoecology 520(2019).