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DOI	10.1016/j.marpolbul.2018.12.011
	Effects and improvements of different reagents preservation methods on the determination of phosphate in seawater by phosphomolybdenum blue spectrophotometric method
	Li Q.; Zhao W.; Miao H.; Han X.
发表日期	2019
ISSN	0025326X
起始页码	136
结束页码	140
卷号	139
英文摘要	This work attempted to prolong the validity of the molybdate mixed solution and ascorbic acid solution used in the phosphomolybdenum blue spectrophotometric method by improving their preservation according to the influence factors. The results showed that the molybdate mixed solution can be directly preserved in darkness with validity over half a year. The ascorbic acid solution is influenced by light, temperature, pH, metal ions, oxygen, and bacteria. The validity of ascorbic acid is shortened as the temperature rises. Through keeping in darkness, adding complexing agents, adjusting pH, removing oxygen and sterilization, the validity of ascorbic acid solution was prolonged to over 2.7 times under 4 °C and over 5 times under 25 °C. At the same time, the hybrid solution of ascorbic acid solution and molybdate mixed solution should be preserved separately, otherwise the using effect is poor. © 2018 Elsevier Ltd
英文关键词	Marine phosphate; Marine phosphate detection; Phosphomolybdenum blue spectrophotometric method; Reagents preservation
语种	英语
scopus关键词	Metal ions; Metals; Oxygen; Seawater effects; Spectrophotometers; Spectrophotometry; Complexing agents; Hybrid solution; Mixed solution; Phosphate detections; Preservation methods; Spectro-photometric method; Temperature rise; Ascorbic acid; ascorbic acid; metal ion; molybdenum; oxygen; phosphate; phosphomolybdenum; reagent; sea water; unclassified drug; dyes, reagents, indicators, markers and buffers; ion; molybdenum; molybdenum blue; phosphate; phosphomolybdic acid; phosphoric acid; sea water; ascorbic acid; detection method; marine pollution; molybdate group; phosphate; preservation; seawater; spectrophotometry; Article; concentration (parameters); controlled study; light; pH; preservation; process optimization; solution and solubility; spectrophotometry; temperature; water content; analysis; chemistry; environmental monitoring; procedures; spectrophotometry; water pollutant; Ascorbic Acid; Environmental Monitoring; Indicators and Reagents; Ions; Molybdenum; Phosphates; Phosphoric Acids; Seawater; Spectrophotometry; Temperature; Water Pollutants, Chemical
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/150157
作者单位	CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, China; Center for Ocean Mega Science, Chinese Academy of Sciences, China; University of Chinese Academy of Sciences, China
推荐引用方式 GB/T 7714	Li Q.,Zhao W.,Miao H.,et al. Effects and improvements of different reagents preservation methods on the determination of phosphate in seawater by phosphomolybdenum blue spectrophotometric method[J],2019,139.
APA	Li Q.,Zhao W.,Miao H.,&Han X..(2019).Effects and improvements of different reagents preservation methods on the determination of phosphate in seawater by phosphomolybdenum blue spectrophotometric method.Marine Pollution Bulletin,139.
MLA	Li Q.,et al."Effects and improvements of different reagents preservation methods on the determination of phosphate in seawater by phosphomolybdenum blue spectrophotometric method".Marine Pollution Bulletin 139(2019).